| Literature DB >> 22310498 |
Patricia Back1, Filip Matthijssens, Jacques R Vanfleteren, Bart P Braeckman.
Abstract
Because superoxide is involved in various physiological processes, many efforts have been made to improve its accurate quantification. We optimized and validated a superoxide-specific and -sensitive detection method. The protocol is based on fluorescence detection of the superoxide-specific hydroethidine (HE) oxidation product, 2-hydroxyethidium. We established a method for the quantification of superoxide production in isolated mitochondria without the need for acetone extraction and purification chromatography as described in previous studies. Copyright ÂEntities:
Mesh：
Substances：
Year: 2012 PMID： 22310498 DOI： 10.1016/j.ab.2012.01.008
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365