BACKGROUND AND AIMS: Elevated levels of circulating microparticles (MPs) have been reported in patients with acute myocardial infarction (AMI) and coronary artery disease. Platelet activation and inflammation have been recognized during AMI and stable angina (SA). We hypothesize that the origin and count of MPs in AMI and SA patients are related to markers of inflammation and platelet activation. METHODS: Platelet, monocytes and endothelial MPs and surface P-selectin were determined in 12 AMI patients, 10 SA patients and 9 controls by flow cytometry. Plasma P-selectin, CD40 ligand (sCD40L) and interleukin 6 (IL-6) levels were evaluated by ELISA methods. RESULTS: The total MP count was compared in control subjects, AMI, and SA patients: 12,765 (8465) vs. 38,750 (11,931) vs. 29,715 (12,072) counts/μl (p = 0.01), respectively. Patients with AMI displayed higher levels of total and platelet origin- tissue factor-positive (CD42/CD142) MPs than patients with SA: 72.8 (6.2) vs. 56.2 (6.4) %, p = 0.001. Levels of soluble P-selectin were significantly elevated in patients with AMI as compared to SA patients: 146 (6.5) vs. 107 (2.7) ng/mL, p = 0.005; significant correlation between total MP count and relative number of CD34, CD51, CD42-positive MPs, and the P-selectin expression was observed in patients with AMI. CONCLUSIONS: Platelet activation in AMI is associated with increased generation of MPs not only from platelets, but also monocytes and endothelial cells. It suggests that interactions between platelets, monocytes and endothelial cells play an important role in the pathogenesis of myocardial ischemia.
BACKGROUND AND AIMS: Elevated levels of circulating microparticles (MPs) have been reported in patients with acute myocardial infarction (AMI) and coronary artery disease. Platelet activation and inflammation have been recognized during AMI and stable angina (SA). We hypothesize that the origin and count of MPs in AMI and SA patients are related to markers of inflammation and platelet activation. METHODS: Platelet, monocytes and endothelial MPs and surface P-selectin were determined in 12 AMI patients, 10 SA patients and 9 controls by flow cytometry. Plasma P-selectin, CD40 ligand (sCD40L) and interleukin 6 (IL-6) levels were evaluated by ELISA methods. RESULTS: The total MP count was compared in control subjects, AMI, and SA patients: 12,765 (8465) vs. 38,750 (11,931) vs. 29,715 (12,072) counts/μl (p = 0.01), respectively. Patients with AMI displayed higher levels of total and platelet origin- tissue factor-positive (CD42/CD142) MPs than patients with SA: 72.8 (6.2) vs. 56.2 (6.4) %, p = 0.001. Levels of soluble P-selectin were significantly elevated in patients with AMI as compared to SA patients: 146 (6.5) vs. 107 (2.7) ng/mL, p = 0.005; significant correlation between total MP count and relative number of CD34, CD51, CD42-positive MPs, and the P-selectin expression was observed in patients with AMI. CONCLUSIONS: Platelet activation in AMI is associated with increased generation of MPs not only from platelets, but also monocytes and endothelial cells. It suggests that interactions between platelets, monocytes and endothelial cells play an important role in the pathogenesis of myocardial ischemia.
Authors: Murad Vagida; Anush Arakelyan; Anna Lebedeva; Jean-Charles Grivel; Alexander Shpektor; Elena Vasilieva; Leonid Margolis Journal: Platelets Date: 2016-09-05 Impact factor: 3.862
Authors: M S Vagida; A Arakelyan; A M Lebedeva; J-Ch Grivel; A V Shpektor; E Yu Vasilieva; L B Margolis Journal: Biochemistry (Mosc) Date: 2016-04 Impact factor: 2.487
Authors: Sauri Hernández-Reséndiz; Mónica Muñoz-Vega; Whendy E Contreras; Gustavo E Crespo-Avilan; Julian Rodriguez-Montesinos; Oscar Arias-Carrión; Oscar Pérez-Méndez; William A Boisvert; Klaus T Preissner; Hector A Cabrera-Fuentes Journal: Cond Med Date: 2018-08
Authors: E Gkaliagkousi; E Gavriilaki; E Yiannaki; I Vasileiadis; B Nikolaidou; A Lazaridis; P Dolgyras; S Grigoriadis; A Triantafyllou; P Anyfanti; D Markala; I Zarifis; S Douma Journal: J Thromb Thrombolysis Date: 2020-10-11 Impact factor: 2.300