Literature DB >> 22301775

Expression of high-affinity human antibody fragments in bacteria.

Romain Rouet1, David Lowe, Kip Dudgeon, Brendan Roome, Peter Schofield, David Langley, John Andrews, Peter Whitfeld, Lutz Jermutus, Daniel Christ.   

Abstract

Here we describe protocols for the expression of human antibody fragments in Escherichia coli. Antigen-specific clones are identified by soluble fragment ELISA and concentrated by periplasmic preparation. They are then further purified by affinity chromatography. This article provides an overview of expression and purification strategies for human antibody fragments, as well as detailed protocols for the identification of high-affinity binders and for affinity maturation.

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Year:  2012        PMID： 22301775     DOI： 10.1038/nprot.2011.448

Source DB:  PubMed          Journal:  Nat Protoc        ISSN： 1750-2799            Impact factor:   13.491


  26 in total

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