Using cultured mammalian cells to study mitosis.

Diverse cell types have been used to study various aspects of mitosis. Early investigators focused primarily on cells that were suited to morphological studies. More recently, experimental systems have been developed to study both morphology and the molecular basis of chromosome motion and cell-cycle regulation. This article briefly reviews cell types that have been used to study mitosis in live cells. It then discusses cell lines that have been used to examine mitosis in cultured mammalian cells and summarizes the methods that are used to culture and study these cells.