| Literature DB >> 2230152 |
A Corti1, P Tripputi, G Cassani.
Abstract
A rapid and simple method for evaluating the efficiency of DNA labelling reactions with haptens is described. The method, called the Flow-Through Hapten-DNA Assay (FT-HDA), relies on binding of anti-hapten antibodies/alkaline phosphatase conjugates to hapten-DNA, immobilized on disposable capillary absorbent filters, and visual detection of blue-grey coloured spots appearing on the filter after chromogenic reaction with enzyme substrates. FT-HDA of hapten-DNA is markedly faster and simpler than conventional diffusion assays on membranes.Mesh：
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Year: 1990 PMID： 2230152 DOI： 10.1016/0022-1759(90)90114-b
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303