Literature DB >> 2229399

Western immunoblot and flagellum enzyme-linked immunosorbent assay for serodiagnosis of Lyme borreliosis.

M Karlsson1.   

Abstract

Western immunoblot with a whole-cell lysate was compared with an enzyme-linked immunosorbent assay with a purified flagellum antigen of Borrelia burgdorferi for serodiagnosis of Lyme borreliosis. The assays showed similar sensitivities and specificities in detecting immunoglobulin M and/or immunoglobulin G antibodies in sera from 68 patients with neuroborreliosis and 44 controls with meningitis and encephalitis or with multiple sclerosis. Flagellum enzyme-linked immunosorbent assay is more easily standardized and seems to be a more suitable diagnostic test in a routine laboratory.

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Year:  1990        PMID： 2229399      PMCID： PMC268127          DOI： 10.1128/jcm.28.9.2148-2150.1990

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  8 in total

1.  Clinical manifestations and diagnosis of neuroborreliosis.

Authors:  G Stiernstedt; R Gustafsson; M Karlsson; B Svenungsson; B Sköldenberg
Journal:  Ann N Y Acad Sci       Date:  1988       Impact factor: 5.691

2.  Measurement of antibodies to the Borrelia burgdorferi flagellum improves serodiagnosis in Lyme disease.

Authors:  K Hansen; P Hindersson; N S Pedersen
Journal:  J Clin Microbiol       Date:  1988-02       Impact factor: 5.948

3.  Quantitative western immunoblotting analysis in survey of human immunodeficiency virus-seropositive patients.

Authors:  D G Schiavini; J M Puel; S A Averous; J A Bazex
Journal:  J Clin Microbiol       Date:  1989-09       Impact factor: 5.948

4.  Comparison of flagellum and sonicate antigens for serological diagnosis of Lyme borreliosis.

Authors:  M Karlsson; G Stiernstedt; M Granström; E Asbrink; B Wretlind
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1990-03       Impact factor: 3.267

5.  Comparison of Western blot and enzyme-linked immunosorbent assay for diagnosis of Lyme borreliosis.

Authors:  M Karlsson; I Möllegård; G Stiernstedt; B Wretlind
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1989-10       Impact factor: 3.267

6.  Comparison of immunoblotting and indirect enzyme-linked immunosorbent assay using different antigen preparations for diagnosing early Lyme disease.

Authors:  R L Grodzicki; A C Steere
Journal:  J Infect Dis       Date:  1988-04       Impact factor: 5.226

7.  Chronic meningitis caused by a penicillin-sensitive microorganism?

Authors:  B Sköldenberg; G Stiernstedt; A Gårde; G Kolmodin; A Carlström; C E Nord
Journal:  Lancet       Date:  1983-07-09       Impact factor: 79.321

8.  Cultivation and characterization of spirochetes from cerebrospinal fluid of patients with Lyme borreliosis.

Authors:  M Karlsson; K Hovind-Hougen; B Svenungsson; G Stiernstedt
Journal:  J Clin Microbiol       Date:  1990-03       Impact factor: 5.948
  8 in total
  15 in total

1.  Immunoblot reactivity of polyclonal and monoclonal antibodies with periplasmic flagellar proteins FlaA1 and FlaB of porcine Serpulina species.

Authors:  L N Fisher; G E Duhamel; R B Westerman; M R Mathiesen
Journal:  Clin Diagn Lab Immunol       Date:  1997-07

2.  Laboratory confirmation of Lyme disease.

Authors:  T G Schwan; W J Simpson; P A Rosa
Journal:  Can J Infect Dis       Date:  1991

3.  Western Blot as a confirmatory test for Lyme disease.

Authors:  H Artsob
Journal:  Can J Infect Dis       Date:  1993-03

4.  Ovalbumin blocking improves sensitivity and specificity of immunoglobulin M immunoblotting for serodiagnosis of patients with erythema migrans.

Authors:  R Lange; H Bocklage; T Schneider; H W Kölmel; J Heesemann; H Karch
Journal:  J Clin Microbiol       Date:  1992-01       Impact factor: 5.948

5.  Enzyme-linked immunosorbent assay using recombinant OspC and the internal 14-kDa flagellin fragment for serodiagnosis of early Lyme disease.

Authors:  S Rauer; N Spohn; C Rasiah; U Neubert; A Vogt
Journal:  J Clin Microbiol       Date:  1998-04       Impact factor: 5.948

6.  Immunoblot interpretation criteria for serodiagnosis of early Lyme disease.

Authors:  S M Engstrom; E Shoop; R C Johnson
Journal:  J Clin Microbiol       Date:  1995-02       Impact factor: 5.948

Review 7.  Tick-borne encephalopathies : epidemiology, diagnosis, treatment and prevention.

Authors:  Göran Günther; Mats Haglund
Journal:  CNS Drugs       Date:  2005       Impact factor: 5.749

8.  Comparison of whole-cell antibodies and an antigenic flagellar epitope of Borrelia burgdorferi in serologic tests for diagnosis of Lyme borreliosis.

Authors:  L A Magnarelli; E Fikrig; R Berland; J F Anderson; R A Flavell
Journal:  J Clin Microbiol       Date:  1992-12       Impact factor: 5.948

9.  Prognostic B-cell epitopes on the flagellar protein of Borrelia burgdorferi.

Authors:  T Schneider; R Lange; W Rönspeck; W Weigelt; H W Kölmel
Journal:  Infect Immun       Date:  1992-01       Impact factor: 3.441

10.  Immunological and molecular polymorphisms of OspC, an immunodominant major outer surface protein of Borrelia burgdorferi.

Authors:  B Wilske; V Preac-Mursic; S Jauris; A Hofmann; I Pradel; E Soutschek; E Schwab; G Will; G Wanner
Journal:  Infect Immun       Date:  1993-05       Impact factor: 3.441
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