Literature DB >> 2229379

Diagnosis of Chlamydia trachomatis cervical infection by detection of amplified DNA with an enzyme immunoassay.

L Bobo1, F Coutlee, R H Yolken, T Quinn, R P Viscidi.   

Abstract

A sensitive and specific system for detection of amplified Chlamydia trachomatis DNA from cervical specimens by fluorometric quantitation in an enzyme immunoassay (EIA) format (polymerase chain reaction [PCR]-EIA) is described. The primers selected for PCR-amplified DNA were from the 15 serovars of C. trachomatis and two strains of Chlamydia pneumoniae (TWAR). One strain of Chlamydia psittaci (Borg) was not amplified. One hundred four previously cultured cervical specimens were evaluated. Forty-six culture-positive specimens containing from 1+ to 4+ inclusion bodies were all positive by PCR-EIA. Of 58 culture-negative specimens, 2 were repeatedly positive and were nonreactive with control probes. This assay system represents a sensitive and specific combination of technologies for the quantitative detection of C. trachomatis DNA directly from a body fluid.

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Year:  1990        PMID： 2229379      PMCID： PMC268088          DOI： 10.1128/jcm.28.9.1968-1973.1990

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  16 in total

1.  Characterization of the new Chlamydia agent, TWAR, as a unique organism by restriction endonuclease analysis and DNA-DNA hybridization.

Authors:  L A Campbell; C C Kuo; J T Grayston
Journal:  J Clin Microbiol       Date:  1987-10       Impact factor: 5.948

2.  Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.

Authors:  R K Saiki; S Scharf; F Faloona; K B Mullis; G T Horn; H A Erlich; N Arnheim
Journal:  Science       Date:  1985-12-20       Impact factor: 47.728

3.  Human immunodeficiency virus type 1 infection in homosexual men who remain seronegative for prolonged periods.

Authors:  D T Imagawa; M H Lee; S M Wolinsky; K Sano; F Morales; S Kwok; J J Sninsky; P G Nishanian; J Giorgi; J L Fahey
Journal:  N Engl J Med       Date:  1989-06-01       Impact factor: 91.245

4.  Detection of multiple, novel reverse transcriptase coding sequences in human nucleic acids: relation to primate retroviruses.

Authors:  A Shih; R Misra; M G Rush
Journal:  J Virol       Date:  1989-01       Impact factor: 5.103

5.  Enzymatic amplification of hepatitis B virus DNA in serum compared with infectivity testing in chimpanzees.

Authors:  P P Ulrich; R A Bhat; B Seto; D Mack; J Sninsky; G N Vyas
Journal:  J Infect Dis       Date:  1989-07       Impact factor: 5.226

6.  Polymerase chain reaction for fast, nonradioactive detection of high- and low-risk papillomavirus types in routine cervical specimens and in biopsies.

Authors:  P B Dallas; J L Flanagan; B N Nightingale; B J Morris
Journal:  J Med Virol       Date:  1989-02       Impact factor: 2.327

7.  Cloning and sequence analysis of the major outer membrane protein genes of two Chlamydia psittaci strains.

Authors:  Y X Zhang; S G Morrison; H D Caldwell; W Baehr
Journal:  Infect Immun       Date:  1989-05       Impact factor: 3.441

8.  Evidence that Chlamydia pneumoniae, strain TWAR, is not sexually transmitted.

Authors:  D K Li; J R Daling; S P Wang; J T Grayston
Journal:  J Infect Dis       Date:  1989-08       Impact factor: 5.226

9.  A sensitive method for the identification of uncharacterized viruses related to known virus groups: hepadnavirus model system.

Authors:  D H Mack; J J Sninsky
Journal:  Proc Natl Acad Sci U S A       Date:  1988-09       Impact factor: 11.205

10.  Specific amplification of a DNA sequence common to all Chlamydia trachomatis serovars using the polymerase chain reaction.

Authors:  B Dutilh; C Bébéar; P Rodriguez; A Vekris; J Bonnet; M Garret
Journal:  Res Microbiol       Date:  1989-01       Impact factor: 3.992
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  47 in total

1.  Confirmatory polymerase chain reaction testing for Chlamydia trachomatis in first-void urine from asymptomatic and symptomatic men.

Authors:  J B Mahony; K E Luinstra; J W Sellors; D Jang; M A Chernesky
Journal:  J Clin Microbiol       Date:  1992-09       Impact factor: 5.948

2.  Two-step polymerase chain reactions and restriction endonuclease analyses detect and differentiate ompA DNA of Chlamydia spp.

Authors:  B Kaltenboeck; K G Kousoulas; J Storz
Journal:  J Clin Microbiol       Date:  1992-05       Impact factor: 5.948

3.  The value of non-culture techniques for diagnosis of Chlamydia trachomatis infections: making the best of a bad job.

Authors:  D Taylor-Robinson
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1992-06       Impact factor: 3.267

4.  Development and validation of a rotor-gene real-time PCR assay for detection, identification, and quantification of Chlamydia trachomatis in a single reaction.

Authors:  Hamid Jalal; Hannah Stephen; Martin D Curran; Janet Burton; Michelle Bradley; Christopher Carne
Journal:  J Clin Microbiol       Date:  2006-01       Impact factor: 5.948

5.  Comparison of polymerase chain reaction and chlamydiazyme for the detection of Chlamydia trachomatis in clinical specimens.

Authors:  T W Williams; S D Tyler; S Giercke; D R Pollard; P McNicol; K R Rozee
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1992-03       Impact factor: 3.267

Review 6.  Polymerase chain reaction: trenches to benches.

Authors:  D H Persing
Journal:  J Clin Microbiol       Date:  1991-07       Impact factor: 5.948

Review 7.  Diagnosis and assessment of trachoma.

Authors:  Anthony W Solomon; Rosanna W Peeling; Allen Foster; David C W Mabey
Journal:  Clin Microbiol Rev       Date:  2004-10       Impact factor: 26.132

8.  Comparison of plasmid- and chromosome-based polymerase chain reaction assays for detecting Chlamydia trachomatis nucleic acids.

Authors:  J B Mahony; K E Luinstra; J W Sellors; M A Chernesky
Journal:  J Clin Microbiol       Date:  1993-07       Impact factor: 5.948

9.  Evaluation of Clearview and Magic Lite tests, polymerase chain reaction, and cell culture for detection of Chlamydia trachomatis in urogenital specimens.

Authors:  J A Kluytmans; W H Goessens; J W Mouton; J H van Rijsoort-Vos; H G Niesters; W G Quint; L Habbema; E Stolz; J H Wagenvoort
Journal:  J Clin Microbiol       Date:  1993-12       Impact factor: 5.948

10.  Use of PCR-enzyme immunoassay for identification of influenza A virus matrix RNA in clinical samples negative for cultivable virus.

Authors:  T Cherian; L Bobo; M C Steinhoff; R A Karron; R H Yolken
Journal:  J Clin Microbiol       Date:  1994-03       Impact factor: 5.948
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