Literature DB >> 2229340

Recombinant fusion protein for simple detection of Escherichia coli heat-stable enterotoxin by GM1 enzyme-linked immunosorbent assay.

J Sanchez1, J Holmgren, A M Svennerholm.   

Abstract

A recombinant gene fusion protein composed of an Escherichia coli heat-stable enterotoxin (STa) peptide epitope fused to the amino end of the cholera toxin B subunit was used to detect STa produced by clinical isolates of enterotoxigenic E. coli (STa-ETEC) by a single monoclonal antibody-based inhibition GM1 enzyme-linked immunosorbent assay. In this test, 100% sensitivity and 100% specificity were observed for use of the recombinant protein in either its purified form or as crude Vibrio cholerae culture supernatants in detection of STa-ETEC.

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Year:  1990        PMID： 2229340      PMCID： PMC268141          DOI： 10.1128/jcm.28.10.2175-2177.1990

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  13 in total

1.  Recombinant system for overexpression of cholera toxin B subunit in Vibrio cholerae as a basis for vaccine development.

Authors:  J Sanchez; J Holmgren
Journal:  Proc Natl Acad Sci U S A       Date:  1989-01       Impact factor: 11.205

2.  Purification of cholera toxin and its subunits: new methods of preparation and the use of hypertoxinogenic mutants.

Authors:  J J Mekalanos; R J Collier; W R Romig
Journal:  Infect Immun       Date:  1978-05       Impact factor: 3.441

3.  Recombinant nontoxinogenic Vibrio cholerae strains as attenuated cholera vaccine candidates.

Authors:  J B Kaper; H Lockman; M M Baldini; M M Levine
Journal:  Nature       Date:  1984 Apr 12-18       Impact factor: 49.962

4.  Biological evaluation of a methanol-soluble, heat-stable Escherichia coli enterotoxin in infant mice, pigs, rabbits, and calves.

Authors:  M N Burgess; R J Bywater; C M Cowley; N A Mullan; P M Newsome
Journal:  Infect Immun       Date:  1978-08       Impact factor: 3.441

5.  Receptor-specific large-scale purification of cholera toxin on silica beads derivatized with lysoGM1 ganglioside.

Authors:  J L Tayot; J Holmgren; L Svennerholm; M Lindblad; M Tardy
Journal:  Eur J Biochem       Date:  1981-01

6.  Detection and characterization of colonization factor of enterotoxigenic Escherichia coli isolated from adults with diarrhea.

Authors:  D G Evans; D J Evans; W S Tjoa; H L DuPont
Journal:  Infect Immun       Date:  1978-02       Impact factor: 3.441

7.  Suckling mouse model for detection of heat-stable Escherichia coli enterotoxin: characteristics of the model.

Authors:  R A Giannella
Journal:  Infect Immun       Date:  1976-07       Impact factor: 3.441

8.  Development of a competitive enzyme-linked immunosorbent assay (ELISA) for Escherichia coli heat-stable enterotoxin (STa).

Authors:  D E Lockwood; D C Robertson
Journal:  J Immunol Methods       Date:  1984-12-31       Impact factor: 2.303

9.  Development of a radioimmunoassay for Escherichia coli heat-stable enterotoxin: comparison with the suckling mouse bioassay.

Authors:  R A Giannella; K W Drake; M Luttrell
Journal:  Infect Immun       Date:  1981-07       Impact factor: 3.441

10.  Enzyme-linked immunosorbent assay for Escherichia coli heat-stable enterotoxin.

Authors:  F A Klipstein; R F Engert; R A Houghten; B Rowe
Journal:  J Clin Microbiol       Date:  1984-06       Impact factor: 5.948
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  9 in total

1.  Purification and characterization of Yersinia enterocolitica and Yersinia pestis LcrV-cholera toxin A(2)/B chimeras.

Authors:  Juliette K Tinker; Chadwick T Davis; Britni M Arlian
Journal:  Protein Expr Purif       Date:  2010-05-11       Impact factor: 1.650

2.  Enterotoxigenic Escherichia coli with STh and STp genotypes is associated with diarrhea both in children in areas of endemicity and in travelers.

Authors:  Ingrid Bölin; Gudrun Wiklund; Firdausi Qadri; Olga Torres; A Louis Bourgeois; Stephen Savarino; Ann-Mari Svennerholm
Journal:  J Clin Microbiol       Date:  2006-08-30       Impact factor: 5.948

3.  Discovery and phylogenetic analysis of novel members of class b enterotoxigenic Escherichia coli adhesive fimbriae.

Authors:  Rania A Nada; Hind I Shaheen; Sami B Khalil; Adel Mansour; Nasr El-Sayed; Iman Touni; Matthew Weiner; Adam W Armstrong; John D Klena
Journal:  J Clin Microbiol       Date:  2011-02-02       Impact factor: 5.948

4.  Case series study of traveler's diarrhea in U.S. military personnel at Incirlik Air Base, Turkey.

Authors:  C K Porter; H El Mohammady; S Baqar; D M Rockabrand; S D Putnam; D R Tribble; M S Riddle; R W Frenck; P Rozmajzl; E Kilbane; A Fox; R Ruck; M Lim; Y J Johnston; E Murphy; J W Sanders
Journal:  Clin Vaccine Immunol       Date:  2008-10-08

5.  Mucosal immunization with a Staphylococcus aureus IsdA-cholera toxin A2/B chimera induces antigen-specific Th2-type responses in mice.

Authors:  Britni M Arlian; Juliette K Tinker
Journal:  Clin Vaccine Immunol       Date:  2011-07-06

6.  A prospective study of acute diarrhea in a cohort of United States military personnel on deployment to the Multinational Force and Observers, Sinai, Egypt.

Authors:  Mark S Riddle; David M Rockabrand; Carey Schlett; Marshall R Monteville; Robert W Frenck; Marcy Romine; Salwa F Ahmed; John W Sanders
Journal:  Am J Trop Med Hyg       Date:  2011-01       Impact factor: 2.345

7.  Diarrhea burden due to natural infection with enterotoxigenic Escherichia coli in a birth cohort in a rural Egyptian community.

Authors:  A Mansour; H I Shaheen; M Amine; K Hassan; J W Sanders; M S Riddle; A W Armstrong; A M Svennerholm; P J Sebeny; J D Klena; S Y N Young; R W Frenck
Journal:  J Clin Microbiol       Date:  2014-05-14       Impact factor: 5.948

8.  Evaluation of a simple, rapid and field-adapted diagnostic assay for enterotoxigenic E. coli and Shigella.

Authors:  Sean Connor; Mirza Velagic; Xueyan Zhang; Fatema-Tuz Johura; Goutam Chowdhury; Asish K Mukhopadhyay; Shanta Dutta; Munirul Alam; David A Sack; Thomas F Wierzba; Subhra Chakraborty
Journal:  PLoS Negl Trop Dis       Date:  2022-02-07

9.  High disease burden of diarrhea due to enterotoxigenic Escherichia coli among rural Egyptian infants and young children.

Authors:  Malla R Rao; Remon Abu-Elyazeed; Stephen J Savarino; Abdollah B Naficy; Thomas F Wierzba; Ibrahim Abdel-Messih; Hind Shaheen; Robert W Frenck; Ann-Mari Svennerholm; John D Clemens
Journal:  J Clin Microbiol       Date:  2003-10       Impact factor: 5.948
  9 in total

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