AIM: The aim of this study was to determine whether remodelling of the desmin (DES) cytoskeleton affects myocardial function and whether it could be a useful marker of disease progression in patients with idiopathic dilated cardiomyopathy (IDCM). MATERIAL AND METHODS: Endomyocardial biopsy was performed in 195 IDCM patients, and five to six specimens were collected from the left ventricle. DES expression was evaluated using tissue immunostaining and Western blotting. The study population was assigned to four groups according to DES expression type: I, normal DES staining at Z-lines giving a regular pattern of cross-striation (n = 57); IIA, increased DES staining with a regular pattern of cross-striation (n = 40); IIB, increased DES staining with an irregular pattern of cross-striation and/or the presence of aggregates (n = 56); and III, decreased/lack of DES staining (n = 42). Fibrosis, cardiomyocyte hypertrophy and ultrastructure were assessed for the four types of DES expression. RESULTS: The pathological types of DES expression (IIB or III) were associated with pathological changes in mitochondria and the contractile apparatus. Cardiomyocyte diameter and level of fibrosis were both significantly affected. DES expression type correlated with NYHA class, left ventricular end-diastolic diameter, left ventricular ejection fraction and the level of N-terminal pro-brain natriuretic protein. CONCLUSION: The type of immunohistochemical DES expression correlated with the level of myocardial injury at the cellular and organ levels. This correlation was similar to that observed between DES expression and the well-established biochemical, echocardiographic and clinical parameters of heart failure (HF). DES expression type could be used as an important diagnostic feature of HF development.
AIM: The aim of this study was to determine whether remodelling of the desmin (DES) cytoskeleton affects myocardial function and whether it could be a useful marker of disease progression in patients with idiopathic dilated cardiomyopathy (IDCM). MATERIAL AND METHODS: Endomyocardial biopsy was performed in 195 IDCM patients, and five to six specimens were collected from the left ventricle. DES expression was evaluated using tissue immunostaining and Western blotting. The study population was assigned to four groups according to DES expression type: I, normal DES staining at Z-lines giving a regular pattern of cross-striation (n = 57); IIA, increased DES staining with a regular pattern of cross-striation (n = 40); IIB, increased DES staining with an irregular pattern of cross-striation and/or the presence of aggregates (n = 56); and III, decreased/lack of DES staining (n = 42). Fibrosis, cardiomyocyte hypertrophy and ultrastructure were assessed for the four types of DES expression. RESULTS: The pathological types of DES expression (IIB or III) were associated with pathological changes in mitochondria and the contractile apparatus. Cardiomyocyte diameter and level of fibrosis were both significantly affected. DES expression type correlated with NYHA class, left ventricular end-diastolic diameter, left ventricular ejection fraction and the level of N-terminal pro-brain natriuretic protein. CONCLUSION: The type of immunohistochemical DES expression correlated with the level of myocardial injury at the cellular and organ levels. This correlation was similar to that observed between DES expression and the well-established biochemical, echocardiographic and clinical parameters of heart failure (HF). DES expression type could be used as an important diagnostic feature of HF development.
Authors: Premi Haynes; Kristofer E Nava; Benjamin A Lawson; Charles S Chung; Mihail I Mitov; Stuart G Campbell; Arnold J Stromberg; Sakthivel Sadayappan; Mark R Bonnell; Charles W Hoopes; Kenneth S Campbell Journal: J Mol Cell Cardiol Date: 2014-02-20 Impact factor: 5.000
Authors: E Czarnowska; D Domal-Kwiatkowska; E Reichman-Warmusz; J B Bierla; A Sowinska; A Ratajska; K Goral-Radziszewska; R Wojnicz Journal: PPAR Res Date: 2016-02-15 Impact factor: 4.964
Authors: Estefanía Tarazón; Miguel Rivera; Esther Roselló-Lletí; Maria Micaela Molina-Navarro; Ignacio José Sánchez-Lázaro; Francisco España; José Anastasio Montero; Francisca Lago; José Ramón González-Juanatey; Manuel Portolés Journal: PLoS One Date: 2012-11-12 Impact factor: 3.240
Authors: Marion Bouvet; Emilie Dubois-Deruy; Annie Turkieh; Paul Mulder; Victoriane Peugnet; Maggy Chwastyniak; Olivia Beseme; Arthur Dechaumes; Philippe Amouyel; Vincent Richard; Nicolas Lamblin; Florence Pinet Journal: Cell Death Discov Date: 2021-06-26