Simplifying native chemical ligation with an N-acylsulfonamide linker.

We report a simplified procedure for the chemical ligation of peptides by using the sulfamylbutyryl linker as a mildly activating group capable of participating in ligation. When the peptidyl N-methylsulfonamide is directly added with excess thiols to ligation reactions, the speed of reaction is comparable to native chemical ligation. This journal is © The Royal Society of Chemistry 2012