J Wang1, B Liu, S Gu, J Liang. 1. Department of Endodontics and Operative Dentistry, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Abstract
OBJECTIVES: The Wnt signalling pathway has been shown to play an important role in tooth development, however its effects with stem cells from the apical papilla (SCAP) have remained unclear. The purpose of this study was to determine effects of Wnt/β-catenin on proliferation and differentiation of SCAP in vitro. MATERIALS AND METHODS: SCAP were obtained, identified and cultured. Cell proliferation, alkaline phosphatase (ALP) activity, mRNA expression of mineralization-related genes and mineralized nodule formation were measured in presence or absence of various concentrations of lithium chloride. RESULTS: MTT assay and flow cytometry demonstrated that Wnt/β-catenin activity could promote proliferation of SCAP. Real-time PCR analysis found that Wnt/β-catenin strongly upregulated expression of dentine sialophosphoprotein, osteocalcin and ALP in SCAP after incubation with mineralization induction medium, while ALP and alizarin red staining indicated that Wnt/β-catenin enhanced ALP activity and formation of mineralized nodules. CONCLUSION: Our results suggest that canonical Wnt/β-catenin signalling promotes proliferation and odonto/osteogenic differentiation of SCAP.
OBJECTIVES: The Wnt signalling pathway has been shown to play an important role in tooth development, however its effects with stem cells from the apical papilla (SCAP) have remained unclear. The purpose of this study was to determine effects of Wnt/β-catenin on proliferation and differentiation of SCAP in vitro. MATERIALS AND METHODS:SCAP were obtained, identified and cultured. Cell proliferation, alkaline phosphatase (ALP) activity, mRNA expression of mineralization-related genes and mineralized nodule formation were measured in presence or absence of various concentrations of lithium chloride. RESULTS:MTT assay and flow cytometry demonstrated that Wnt/β-catenin activity could promote proliferation of SCAP. Real-time PCR analysis found that Wnt/β-catenin strongly upregulated expression of dentine sialophosphoprotein, osteocalcin and ALP in SCAP after incubation with mineralization induction medium, while ALP and alizarin red staining indicated that Wnt/β-catenin enhanced ALP activity and formation of mineralized nodules. CONCLUSION: Our results suggest that canonical Wnt/β-catenin signalling promotes proliferation and odonto/osteogenic differentiation of SCAP.
Authors: Frederic Morvan; Kim Boulukos; Philippe Clément-Lacroix; Sergio Roman Roman; Isabelle Suc-Royer; Béatrice Vayssière; Patrick Ammann; Patrick Martin; Sonia Pinho; Philippe Pognonec; Patrick Mollat; Christof Niehrs; Roland Baron; Georges Rawadi Journal: J Bone Miner Res Date: 2006-06 Impact factor: 6.741
Authors: Jee Hyun An; Jae-Yeon Yang; Byung Yong Ahn; Sun Wook Cho; Ju Yeon Jung; Hwa Young Cho; Young Min Cho; Sang Wan Kim; Kyong Soo Park; Seong Yeon Kim; Hong Kyu Lee; Chan Soo Shin Journal: Bone Date: 2010-04-14 Impact factor: 4.398
Authors: Changyong Yuan; Penglai Wang; Lifang Zhu; Waruna Lakmal Dissanayaka; David William Green; Edith H Y Tong; Lijian Jin; Chengfei Zhang Journal: Tissue Eng Part A Date: 2014-12-23 Impact factor: 3.845