BACKGROUND: Tescalcin is an EF-hand calcium-binding protein that interacts with the Na+/H+ exchanger 1 (NHE1). Levay and Slepak recently proposed a role for tescalcin in megakaryopoiesis that was independent of NHE1 activity. Their studies using K562 and HEL cell lines, and human CD34+ hematopoietic stem cells suggested an essential role for tescalcin in megakaryocyte differentiation. OBJECTIVE: To study the role of tescalcin in megakaryocyte development using a murine model of megakaryopoiesis. METHODS: We generated a mouse with targeted disruption of tescalcin and investigated megakaryocyte development. RESULTS: Tescalcin-deficient mice had a normal number of megakaryocytes and platelets. The morphology, polyploidization profile, and expression of Fli-1 in bone marrow-derived megakaryocytes were also normal. CONCLUSION: Tescalcin does not appear to be necessary for normal megakaryocyte development. Copyright Â
BACKGROUND:Tescalcin is an EF-hand calcium-binding protein that interacts with the Na+/H+ exchanger 1 (NHE1). Levay and Slepak recently proposed a role for tescalcin in megakaryopoiesis that was independent of NHE1 activity. Their studies using K562 and HEL cell lines, and humanCD34+ hematopoietic stem cells suggested an essential role for tescalcin in megakaryocyte differentiation. OBJECTIVE: To study the role of tescalcin in megakaryocyte development using a murine model of megakaryopoiesis. METHODS: We generated a mouse with targeted disruption of tescalcin and investigated megakaryocyte development. RESULTS:Tescalcin-deficient mice had a normal number of megakaryocytes and platelets. The morphology, polyploidization profile, and expression of Fli-1 in bone marrow-derived megakaryocytes were also normal. CONCLUSION:Tescalcin does not appear to be necessary for normal megakaryocyte development. Copyright Â
Authors: Ksenia G Kolobynina; Valeria V Solovyova; Konstantin Levay; Albert A Rizvanov; Vladlen Z Slepak Journal: J Cell Sci Date: 2017-01-15 Impact factor: 5.285
Authors: Ksenia G Kolobynina; Valeria V Solovyova; Konstantin Levay; Albert A Rizvanov; Vladlen Z Slepak Journal: J Cell Sci Date: 2016-09-08 Impact factor: 5.285
Authors: Katie L Ayers; Luke S Lambeth; Nadia M Davidson; Andrew H Sinclair; Alicia Oshlack; Craig A Smith Journal: BMC Genomics Date: 2015-09-16 Impact factor: 3.969