L Portillo-Gómez1, E G Sosa-Iglesias. 1. Laboratorio de Microbiología y Parasitología, Investigación en Microbiología Médica, Departamento de Microbiología y Patología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, México.
Abstract
SETTING: The epidemiology of zoonotic tuberculosis (ZTB) in humans in Mexico is poorly known. OBJECTIVE: To identify isolates of Mycobacterium bovis in humans and cattle by polymerase chain reaction (PCR), and establish the clinical and epidemiological importance of ZTB in humans. DESIGN: From 1995 to 2009, 124 isolates from patients with TB and 60 isolates from cattle were analysed. PCR identification was performed using the oxy R gene, and the clinical and epidemiological aspects of ZTB in humans were investigated. RESULTS: PCR identified 93 M. bovis isolates: 35 (28%) from the 124 human isolates and 58 (97%) from the 60 cattle isolates. The sensitivity and specificity of the method were 100%. ZTB in the 35 patients presented as extra-pulmonary TB (EPTB) in 74%: 51% were children, 69% had malnutrition, 51% had consumed unpasteurised milk and 6% had contact with animals; 11% were relapses and 31% died. CONCLUSIONS: PCR using the oxy R gene is highly sensitive, specific and rapid for the identification of M. bois. ZTB is a serious public health problem, and presented as EPTB in children with malnutrition and those who had consumed unpasteurised milk. ZTB provokes relapses and a high mortality rate.
SETTING: The epidemiology of zoonotic tuberculosis (ZTB) in humans in Mexico is poorly known. OBJECTIVE: To identify isolates of Mycobacterium bovis in humans and cattle by polymerase chain reaction (PCR), and establish the clinical and epidemiological importance of ZTB in humans. DESIGN: From 1995 to 2009, 124 isolates from patients with TB and 60 isolates from cattle were analysed. PCR identification was performed using the oxy R gene, and the clinical and epidemiological aspects of ZTB in humans were investigated. RESULTS: PCR identified 93 M. bovis isolates: 35 (28%) from the 124 human isolates and 58 (97%) from the 60 cattle isolates. The sensitivity and specificity of the method were 100%. ZTB in the 35 patients presented as extra-pulmonary TB (EPTB) in 74%: 51% were children, 69% had malnutrition, 51% had consumed unpasteurised milk and 6% had contact with animals; 11% were relapses and 31% died. CONCLUSIONS: PCR using the oxy R gene is highly sensitive, specific and rapid for the identification of M. bois. ZTB is a serious public health problem, and presented as EPTB in children with malnutrition and those who had consumed unpasteurised milk. ZTB provokes relapses and a high mortality rate.
Authors: Ana Laura Guillén-Nepita; Gerardo Vázquez-Marrufo; Andrés Cruz-Hernández; Felipe García-Oliva; Reyna Cristina Zepeda-Gurrola; Ma Soledad Vázquez-Garcidueñas Journal: Pathog Glob Health Date: 2020-09-14 Impact factor: 2.894
Authors: Hawult Taye; Kassahun Alemu; Adane Mihret; James L N Wood; Ziv Shkedy; Stefan Berg; Abraham Aseffa Journal: Zoonoses Public Health Date: 2021-06-24 Impact factor: 2.702
Authors: Rebuma Firdessa; Stefan Berg; Elena Hailu; Esther Schelling; Balako Gumi; Girume Erenso; Endalamaw Gadisa; Teklu Kiros; Meseret Habtamu; Jemal Hussein; Jakob Zinsstag; Brian D Robertson; Gobena Ameni; Amanda J Lohan; Brendan Loftus; Iñaki Comas; Sebastien Gagneux; Rea Tschopp; Lawrence Yamuah; Glyn Hewinson; Stephen V Gordon; Douglas B Young; Abraham Aseffa Journal: Emerg Infect Dis Date: 2013-03 Impact factor: 6.883