A possible regulation mechanism of water content in human stratum corneum via intercellular lipid matrix.

We studied the water regulation mechanism in human stratum corneum which is composed of corneocytes and intercellular lipid matrix by the ex vivo small- and medium-angle X-ray diffraction. Under the normal condition water molecules are stored mainly in the corneocytes. When the water content increased, from the small-angle X-ray diffraction of the human stratum corneum we obtained the swelling behavior of the short lamellar lipid structure as a result of incorporating a very small amount of water into water layers between neighboring the lipid bilayers, and its diffraction peak width became narrow and turned to wide at the water content of 20-30wt%. In addition as evidence for uptake of water in the corneocytes, we observed the structural modification of soft keratins in the corneocytes from the medium-angle X-ray diffraction. Based upon these results we propose that the water content in the human stratum corneum is regulated to be at 20-30wt% so as to stabilize the short lamellar structure in the intercellular lipid matrix. Copyright Â