OBJECTIVE: To study the relationship between the polymorphisms of interleukin-10 gene at position--1082,-592 (IL-10-1082, IL-10-592) and chronic HBV and/or HCV infection. METHODS: 277 subjects (79 chronic HCV/HBV co-infection,69 chronic HBV infection, 55 chronic HCV infection and 74 control subjects) were recruited from plasma donors in a rural area of Hebei Province, China. The single nucleotide polymorphism of IL-10-1082 and IL-10-592 was investigated by sequence specific primer-PCR (SSP-PCR) and restricted fragment long polymorphism-PCR (RFLP-PCR). Hepatocellular injury was detected by alanine aminotransferase (ALT) with Beckman LX-20 analyzer. The presence of hepatitis C viral particles in serum was determined by RT-nPCR. RESULTS: (1) Chronic HCV or HBV infection or HCV/HBV co-infection was associated with higher frequency of IL-10-1082 AA genotype [chi2 = 13.05, P = 0.04; OR = 2.29 (1.10-4.78), 2.34 (1.07-5.10), 2.56 (1.25 -5.21)]. There was no significant association of IL-10-1082 allele frequency with HCV or HBV infection or HCV/HBV co-infections (chi2 = 4.65, P = 0.20). There was no significant association of IL-10-592 genotype and allele frequency with chronic HBV and/or HCV infection (chi2 = 2.83, P = 0.83; chi2 = 1.10, P = 0.78) (2) There were obvious associations of higher IL-10-1082 AA genotype and A allele frequency with HCV replication [(chi2 = 12.27, P = 0.00; chi2 = 5.36, P = 0.02), OR = 3.36 (1.67-6.76) and 1.67 (1.08-2.57)]. The polymorphism of IL-10592 was not associated with HCV RNA replication (chi2 = 2.10, P = 0.35; chi2 = 1.88, P = 0.17). (3) The polymorphism of IL-10-1082 was not significantly associated with abnormal ALT (chi2 = 3.25, P = 0.20; chi2 = 1.79, P = 0.18). Abnormal serum ALT level was associated with IL-10-592 AC genotype [(chi2 = 6.32, P = 0.04), OR = 2.83 (1.26- 6.37)), but the IL-10-592 A/C allele frequency was not associated with abnormal serum ALT level (chi2 = 2.30, P = 0.09). CONCLUSION: These results suggested that the polymorphism of IL-10-1082 appears to have some influences on the chronic infection of HCV and/or HBV and HCV replication. IL-10-592 AC genotype frequency has influence on inflammatory liver injury.
OBJECTIVE: To study the relationship between the polymorphisms of interleukin-10 gene at position--1082,-592 (IL-10-1082, IL-10-592) and chronic HBV and/or HCV infection. METHODS: 277 subjects (79 chronic HCV/HBV co-infection,69 chronic HBV infection, 55 chronic HCV infection and 74 control subjects) were recruited from plasma donors in a rural area of Hebei Province, China. The single nucleotide polymorphism of IL-10-1082 and IL-10-592 was investigated by sequence specific primer-PCR (SSP-PCR) and restricted fragment long polymorphism-PCR (RFLP-PCR). Hepatocellular injury was detected by alanine aminotransferase (ALT) with Beckman LX-20 analyzer. The presence of hepatitis C viral particles in serum was determined by RT-nPCR. RESULTS: (1) Chronic HCV or HBV infection or HCV/HBV co-infection was associated with higher frequency of IL-10-1082 AA genotype [chi2 = 13.05, P = 0.04; OR = 2.29 (1.10-4.78), 2.34 (1.07-5.10), 2.56 (1.25 -5.21)]. There was no significant association of IL-10-1082 allele frequency with HCV or HBV infection or HCV/HBV co-infections (chi2 = 4.65, P = 0.20). There was no significant association of IL-10-592 genotype and allele frequency with chronic HBV and/or HCV infection (chi2 = 2.83, P = 0.83; chi2 = 1.10, P = 0.78) (2) There were obvious associations of higher IL-10-1082 AA genotype and A allele frequency with HCV replication [(chi2 = 12.27, P = 0.00; chi2 = 5.36, P = 0.02), OR = 3.36 (1.67-6.76) and 1.67 (1.08-2.57)]. The polymorphism of IL-10592 was not associated with HCV RNA replication (chi2 = 2.10, P = 0.35; chi2 = 1.88, P = 0.17). (3) The polymorphism of IL-10-1082 was not significantly associated with abnormal ALT (chi2 = 3.25, P = 0.20; chi2 = 1.79, P = 0.18). Abnormal serum ALT level was associated with IL-10-592 AC genotype [(chi2 = 6.32, P = 0.04), OR = 2.83 (1.26- 6.37)), but the IL-10-592 A/C allele frequency was not associated with abnormal serum ALT level (chi2 = 2.30, P = 0.09). CONCLUSION: These results suggested that the polymorphism of IL-10-1082 appears to have some influences on the chronic infection of HCV and/or HBV and HCV replication. IL-10-592 AC genotype frequency has influence on inflammatory liver injury.