Literature DB >> 22278756

MsLDR-creator: a web service to design msLDR assays.

Felix Bormann1, Andreas Dahl, Christine Sers.   

Abstract

MsLDR-creator is a free web service to design assays for the new DNA methylation detection method msLDR. The service provides the user with all necessary information about the oligonucleotides required for the measurement of a given CpG within a sequence of interest. The parameters are calculated by the nearest neighbour approach to achieve optimal behaviour during the experimental procedure. In addition, to guarantee a good start using msLDR, further information, like protocols and hints and tricks, are provided.

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Year:  2012        PMID： 22278756     DOI： 10.1007/s00438-012-0677-9

Source DB:  PubMed          Journal:  Mol Genet Genomics        ISSN： 1617-4623            Impact factor:   3.291


  5 in total

1.  Ligation detection reaction-TaqMan procedure for single nucleotide polymorphism detection on genomic DNA.

Authors:  Tatiana A Borodina; Hans Lehrach; Aleksey V Soldatov
Journal:  Anal Biochem       Date:  2004-10-15       Impact factor: 3.365

2.  Primer design for large scale sequencing.

Authors:  S Haas; M Vingron; A Poustka; S Wiemann
Journal:  Nucleic Acids Res       Date:  1998-06-15       Impact factor: 16.971

3.  Improved thermodynamic parameters and helix initiation factor to predict stability of DNA duplexes.

Authors:  N Sugimoto; S Nakano; M Yoneyama; K Honda
Journal:  Nucleic Acids Res       Date:  1996-11-15       Impact factor: 16.971

4.  A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics.

Authors:  J SantaLucia
Journal:  Proc Natl Acad Sci U S A       Date:  1998-02-17       Impact factor: 11.205

5.  Methylation-specific ligation detection reaction (msLDR): a new approach for multiplex evaluation of methylation patterns.

Authors:  Felix Bormann; Christine Sers; Barbara Seliger; Diana Handke; Thomas Bergmann; Stephanie Seibt; Hans Lehrach; Andreas Dahl
Journal:  Mol Genet Genomics       Date:  2011-08-31       Impact factor: 3.291
  5 in total

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