Stefan G Michalski1, Walter Durka. 1. Department of Community Ecology (BZF), Helmholtz Centre for Environmental Research UFZ, Theodor-Lieser-Strasse 4, D-06120 Halle, Germany. stefan.michalski@ufz.de
Abstract
PREMISE OF THE STUDY: Microsatellite markers were developed for the common wetland plant Juncus effusus to assess levels of within- and among-population genetic diversity. METHODS AND RESULTS: Using a next-generation sequencing approach we identified new microsatellite loci, from which 23 were further characterized in a large population of J. effusus. Expected heterozygosity ranged from 0 to 0.64 with a mean of 0.407. Inbreeding coefficients (mean = 0.984) were very high, confirming earlier suggestions of an inbred mating system. Cross-amplification was tested in J. conglomeratus, J. inflexus, J. drummondii, and J. filiformis, with best results in the former. CONCLUSIONS: The described microsatellite markers will be helpful in assisting studies on, e.g., reproduction, taxonomy, and phylogeography in J. effusus and related species.
PREMISE OF THE STUDY: Microsatellite markers were developed for the common wetland plant Juncus effusus to assess levels of within- and among-population genetic diversity. METHODS AND RESULTS: Using a next-generation sequencing approach we identified new microsatellite loci, from which 23 were further characterized in a large population of J. effusus. Expected heterozygosity ranged from 0 to 0.64 with a mean of 0.407. Inbreeding coefficients (mean = 0.984) were very high, confirming earlier suggestions of an inbred mating system. Cross-amplification was tested in J. conglomeratus, J. inflexus, J. drummondii, and J. filiformis, with best results in the former. CONCLUSIONS: The described microsatellite markers will be helpful in assisting studies on, e.g., reproduction, taxonomy, and phylogeography in J. effusus and related species.
Authors: Muhammad Arslan; Upendra Kumar Devisetty; Martin Porsch; Ivo Große; Jochen A Müller; Stefan G Michalski Journal: BMC Genomics Date: 2019-06-13 Impact factor: 3.969