BACKGROUND: Nitric oxide (NO) is implied in many important biological processes in all metazoans from porifera to chordates. In the cuttlefish Sepia officinalis NO plays a key role in the defense system and neurotransmission. RESULTS: Here, we detected for the first time NO, NO synthase (NOS) and transcript levels during the development of S. officinalis. The spatial pattern of NO and NOS is very dynamic, it begins during organogenesis in ganglia and epithelial tissues, as well as in sensory cells. At later stages, NO and NOS appear in organs and/or structures, including Hoyle organ, gills and suckers. Temporal expression of NOS, followed by real-time PCR, changes during development reaching the maximum level of expression at stage 26. CONCLUSIONS: Overall these data suggest the involvement of NO during cuttlefish development in different fundamental processes, such as differentiation of neural and nonneural structures, ciliary beating, sensory cell maintaining, and organ functioning.
BACKGROUND:Nitric oxide (NO) is implied in many important biological processes in all metazoans from porifera to chordates. In the cuttlefish Sepia officinalis NO plays a key role in the defense system and neurotransmission. RESULTS: Here, we detected for the first time NO, NO synthase (NOS) and transcript levels during the development of S. officinalis. The spatial pattern of NO and NOS is very dynamic, it begins during organogenesis in ganglia and epithelial tissues, as well as in sensory cells. At later stages, NO and NOS appear in organs and/or structures, including Hoyle organ, gills and suckers. Temporal expression of NOS, followed by real-time PCR, changes during development reaching the maximum level of expression at stage 26. CONCLUSIONS: Overall these data suggest the involvement of NO during cuttlefish development in different fundamental processes, such as differentiation of neural and nonneural structures, ciliary beating, sensory cell maintaining, and organ functioning.
Authors: Graziano Fiorito; Andrea Affuso; David B Anderson; Jennifer Basil; Laure Bonnaud; Giovanni Botta; Alison Cole; Livia D'Angelo; Paolo De Girolamo; Ngaire Dennison; Ludovic Dickel; Anna Di Cosmo; Carlo Di Cristo; Camino Gestal; Rute Fonseca; Frank Grasso; Tore Kristiansen; Michael Kuba; Fulvio Maffucci; Arianna Manciocco; Felix Christopher Mark; Daniela Melillo; Daniel Osorio; Anna Palumbo; Kerry Perkins; Giovanna Ponte; Marcello Raspa; Nadav Shashar; Jane Smith; David Smith; António Sykes; Roger Villanueva; Nathan Tublitz; Letizia Zullo; Paul Andrews Journal: Invert Neurosci Date: 2014-01-03
Authors: Fangmin Chen; Benjamin C Krasity; Suzanne M Peyer; Sabrina Koehler; Edward G Ruby; Xiaoping Zhang; Margaret J McFall-Ngai Journal: mBio Date: 2017-04-04 Impact factor: 7.867