Uterine Carcinosarcoma in a 2-year-old Female Wistar Hannover GALAS Rat.

Carcinosarcomas are rare tumors in humans as well as rats and most commonly occur in the uterus. Recently, we observed a case of incidental carcinosarcoma of the uterus in a female Wistar Hannover GALAS [BrlHan:WIST@ Jcl (GALAS)] rat at 2 years of age. Histopathologically, the tumor was characterized by an admixture of malignant epithelial and nonepithelial elements. The carcinomatous components represented a type of endometrial carcinoma, consisting of glandular and solid proliferation of large-sized tumor cells. Prominent mitoses and tumor cell invasion were observed. The sarcomatous components were characterized by multifocal proliferation of severe atypical cells with cartilage matrix and were diagnosed as chondrosarcoma. Transitions between carcinomatous and sarcomatous components were observed, and many tumor cells in the solid lesion showed immunohistochemical reactivity with both cytokeratin and vimentin. Based on these findings, this tumor was diagnosed as a uterine carcinosarcoma. This is the first report of uterine carcinosarcoma in Wistar Hannover GALAS [BrlHan:WIST@Jcl (GALAS)] rats.

Carcinosarcomas are rare tumors that most commonly develop in the female genital tract, particularly in the uterus. Characteristic histopathological findings are coexistence of both epithelial and nonepithelial malignant components. Development of carcinosarcomas has been demonstrated in various human organs, including the uterus, ovary, breast, lung and gastrointestinal tract. Although spontaneous carcinosarcomas in the uterus of LEWIS rats and mammary gland of SD rats have been documented, no reports about this tumor type in Wistar Hannover GALAS rats have been published. In this article, we report a case of carcinosarcoma of the uterus in a female Wistar Hannover GALAS rat at 2 years of age.

In this study, Wistar Hannover GALAS [BrlHan: WIST@Jcl (GALAS)] rats (CLEA Japan, Tokyo, Japan) were randomly allocated to three groups, each consisting of 50 males and 50 females, and groups of rats of each sex were fed a tocotrienol-containing basal diet (CE-2; CLEA Japan, Tokyo, Japan) and tap water ad libitum for 104 weeks beginning at 6 weeks of age. The tocotrienol dose levels in the diet in the high-dose group were 2.0% during weeks 1–50 and 1.0% for the remainder of the study. The tocotrienol (Eisai, Tokyo, Japan) given in this study was composed of α-tocotrienol (21.4%), β-tocotrienol (3.5%), γ-tocotrienol (36.5%), δ-tocotrienol (8.6%), α-tocopherol (20.5%), βtocopherol (0.7%), γ-tocopherol (1.0%) and δ-tocopherol (0.5%). The animals were housed in a clear polycarbonate cage with sterilized white wood chips (Sankyo Labo Service Corporation, Tokyo, Japan) for bedding in a standard air-conditioned room (24 ± 1 °C, 55 ± 5% relative humidity, 12 h light/dark cycles). At the end of experiment, we found one animal with a large mass (1.0 × 0.9 × 0.8 cm) in the left horn of the uterus within the 47 animals in the high-dose group. The mass was dissected with the uterus and fixed in 10% neutral buffered formalin. Macroscopically, nodules were also observed in the pituitary gland and pectoral sub cutis. After fixation, the tissues were routinely processed, i.e., embedded in paraffin, sectioned at 4 µm and stained with hematoxylin and eosin (HE). Additionally, toluidine blue stain and immunohistochemical staining for rabbit anti-cow S-100 protein (DAKO, Glostrup, Denmark), antihuman cytokeratin (clones AE1/AE3; DAKO), anti-porcine vimentin (clone V9; DAKO) and anti-human E-cadherin (clone 36; BD Biosciences, Franklin Lakes, NJ, USA) were performed using serial sections. The animal experimental protocol was reviewed and approved by the Animal Care and Use Committee of the National Institute of Health Sciences, Japan.

Histopathologically, a neoplastic nodule appeared to protrude into the lumen of the uterus (Fig 1a, b). Invasion of tumor cells from the myometrium into the serosa were observed (Fig 1c). Two distinct types of tumor cells were observed in this nodule (Fig 1d). The first component was characterized by a proliferative pattern of tumor cells in irregular gland-like, solid sheets or nests. This type of tumor cell had large round or elliptical nuclei with a few apparent nucleoli and scant or moderate and eosinophilic or basophilic cytoplasm (Fig 2a-c), indicating endometrial carcinoma. Severe necrosis, mineralization and multiple mitoses were observed. Tumor cells with abundant collagen invaded from the myometrium into the serosa and were either single cells or clusters of a few cells (Fig 2d). Immunohistochemically, the tumor cells showing a glandular pattern were strongly positive for cytokeratin and E-cadherin and negative for vimentin, while those with a solid pattern were weakly positive for cytokeratin, partially positive for E-cadherin and mostly positive for vimentin (Fig 2e, f). The second component, which was characterized by a proliferation of polymorphic tumor cells with a pale basophilic matrix, was focally observed within the carcinomatous component. This type of tumor cell had round or oval nuclei with severe atypia and an appreciable amount of clear to eosinophilic cytoplasm (Fig 3a, b). The pale basophilic matrix exhibited metachromasia by toluidine blue staining, indicating cartilage matrix. These tumor cells were negative for E-cadherin, weakly positive for cytokeratin and intensely positive for vimentin and S-100 protein (Fig 3c, d). Thus, this sarcomatous component was diagnosed as chondrosarcoma. Transitions between carcinomatous and sarcomatous components were observed (Fig 1a, d). Although myometrial and vascular in-scopically and microscopically, carcinosarcomas were not vasion and serosal involvement were observed, no metas-observed in other animals including the tocotrienol-treated tasis was seen. Primary carcinomas were also seen in the pituitary gland and mammary gland of this animal. Macroscopically and microscopically, carcinosarcomas were not observed in other animals including the tocotrienol-treated and control animals.

Fig. 1.

Uterine carcinosarcoma. (a) Overall picture of the uterine carcinosarcoma. HE stain. Bar=2 mm. (b) High magnification of the region enclosed in the large square in a. The upper half is an existing uterine area. The arrowheads indicate existing endometrium and uterine glands. The lower half is the tumor area. HE stain. Bar=400 µm. (c) High magnification of the region enclosed in the small square in a. Invasion of tumor cells is observed from the myometrium into the serosa. The arrows indicate the myometrium. HE stain. Bar=200 µm. (d) Epithelial and nonepithelial malignant components are observed in a nodule. HE stain. Bar=200 µm.

Fig. 2.

Histopathological and immunohistochemical findings of carcinomatous elements in the uterine carcinosarcoma. (a) Irregular glandular structures and solid sheet structures of tumor cells. HE stain. Bar=200 µm. (b) High magnification of glandular area in a. Tumor cells have large round or elliptical nuclei and moderate eosinophilic cytoplasm. HE stain. Bar=50 µm. (c) High magnification of the solid sheet area in a. Tumor cells have large round or oval nuclei and scant basophilic cytoplasm. Mitotic figures are observed (arrowhead). HE stain. Bar=50 µm. (d) Invasion of tumor cells with collagen is observed from the myometrium into the serosa. Many small glandular structures are seen within the tumor cells. The arrow indicates the myometrium. HE stain. Bar=200 µm. (e) Immunoreactivity for cytokeratin. Glandular tumor cells are positive, while solid sheet tumor cells are weakly positive. Bar=100 µm. (f) Immunoreactivity for vimentin. Glandular tumor cells are negative, while many of the tumor cells in the solid sheet area are positive (arrowhead). Bar=100 µm.

Fig. 3.

Histopathological and immunohistochemical findings of sarcomatous elements in the uterine carcinosarcoma. (a) Proliferation of tumor cells with a basophilic matrix. HE stain. Bar=100 µm. (b) High magnification of a. Tumor cells have round or oval nuclei and an appreciable amount of eosinophilic cytoplasm. HE stain. Bar=50 µm. (c) Immunoreactivity for cytokeratin. Tumor cells of epithelial origin are positive, while those of sarcomatous components are weakly positive. Bar=100 µm. (d) Immunoreactivity for vimentin. Tumor cells of epithelial origin are negative, while those of sarcomatous components are intensely positive. Bar=100 µm.

Based on the present histopathological and immunohistochemical findings, this tumor was diagnosed as a uterine carcinosarcoma. The conclusive histopathological findings supporting this diagnosis were the mixture of carcinomatous and sarcomatous elements in a single neoplastic nodule. This case was further subclassified as the heterologous type because the sarcomatous components showed typical chondrosarcoma that had developed from an element that did not originally exist. Although carcinosarcomas are unusual tumors, they are the most common type of mixed epithelial-nonepithelial endometrial tumors. The prognosis of carcinosarcoma is generally poor due to recurrence, lymph node metastasis and myometrial and lymph-vascular space invasion. Although metastasis to pelvic or periaortic lymph nodes has been reported, metastasis of tumor cells was not observed in the mesenteric lymph nodes or other organs such as the lung, liver and spleen in the present case. Immunohistochemical staining for cytokeratin, a general epithelial marker, and vimentin, a typical mesenchymal marker, have suggested distinct carcinomatous and sarcomatous populations in carcinosarcomas. The present immunohistochemical findings were consistent with those of the previous reports. Epithelial markers are frequently expressed by sarcomatous-appearing cells, and epithelial and mesenchymal markers are coexpressed in many tumor cells in carcinosarcomas. Carcinosarcomas have been suggested to result from neoplastic transformation of cells with a capacity for both epithelial and mesenchymal differentiation. The histogenesis of carcinosarcomas has been hypothesized to involve a collision tumor, combination/conversion tumor or other compositional tumor. The presence of many carcinosarcomas has been reported to represent a combination tumor. Although we did not perform a stem cell analysis, the present case may be considered to be a combination tumor based on the results of immunohistochemistry and the evidence of mixed and/or transitional pattern of carcinomatous and sarcomatous components.

Several nomenclature systems are used to describe this type of neoplastic lesion. According to the WHO classification, malignant mesodermal mixed tumor is synonymous with carcinosarcoma, but tumors with heterologous and homologous sarcomatous components are sometimes diagnosed as malignant mesodermal mixed tumor and carcinosarcoma, respectively.

The tumor in the present case was detected in only a single rat within a group of similarly treated animals. Therefore, this tumor could be incidental and may not be related to the treatment. This is the first report of uterine carcinosarcoma in a Wistar Hannover GALAS rat.