OBJECTIVES: To identify the role of single nucleotide polymorphisms (SNPs) of the tumor necrosis factor (TNF) gene in the natural course of 2009 influenza A H1N1 virus infection. METHODS: Genomic DNA was isolated from 109 patients with an H1N1 infection and from 108 healthy volunteers. SNPs of the TNF gene were assessed after electrophoresis of the digested PCR products by restriction enzymes. RESULTS: The frequency of the -238 A allele was significantly greater among patients than among controls. Viral pneumonia developed in 20 of 96 non-carriers of at least one -238 A allele (20.8%) and in seven of 13 carriers of at least one -238 A allele (53.8%, p=0.016). Logistic regression analysis showed that the most important factors associated with the development of pneumonia were the presence of an underlying disease (p=0.021, odds ratio (OR) 3.08) and the carriage of at least one -238 A allele (p=0.041, OR 3.74). Gene transcripts of the TNF gene were greater among non-carriers of the -238 A allele than among carriers of the -238 A allele. CONCLUSIONS: The -238 A SNP allele of the TNF gene imposes on the course of 2009 H1N1 virus infection and is an independent risk factor for pneumonia.
OBJECTIVES: To identify the role of single nucleotide polymorphisms (SNPs) of the tumor necrosis factor (TNF) gene in the natural course of 2009 influenza A H1N1 virus infection. METHODS: Genomic DNA was isolated from 109 patients with an H1N1infection and from 108 healthy volunteers. SNPs of the TNF gene were assessed after electrophoresis of the digested PCR products by restriction enzymes. RESULTS: The frequency of the -238 A allele was significantly greater among patients than among controls. Viral pneumonia developed in 20 of 96 non-carriers of at least one -238 A allele (20.8%) and in seven of 13 carriers of at least one -238 A allele (53.8%, p=0.016). Logistic regression analysis showed that the most important factors associated with the development of pneumonia were the presence of an underlying disease (p=0.021, odds ratio (OR) 3.08) and the carriage of at least one -238 A allele (p=0.041, OR 3.74). Gene transcripts of the TNF gene were greater among non-carriers of the -238 A allele than among carriers of the -238 A allele. CONCLUSIONS: The -238 A SNP allele of the TNF gene imposes on the course of 2009 H1N1 virus infection and is an independent risk factor for pneumonia.
Authors: Ying Qin; Peter W Horby; Tim K Tsang; Enfu Chen; Lidong Gao; Jianming Ou; Tran Hien Nguyen; Tran Nhu Duong; Viktor Gasimov; Luzhao Feng; Peng Wu; Hui Jiang; Xiang Ren; Zhibin Peng; Sa Li; Ming Li; Jiandong Zheng; Shelan Liu; Shixiong Hu; Rongtao Hong; Jeremy J Farrar; Gabriel M Leung; George F Gao; Benjamin J Cowling; Hongjie Yu Journal: Clin Infect Dis Date: 2015-05-04 Impact factor: 9.079
Authors: Koldo Garcia-Etxebarria; María Alma Bracho; Juan Carlos Galán; Tomàs Pumarola; Jesús Castilla; Raúl Ortiz de Lejarazu; Mario Rodríguez-Dominguez; Inés Quintela; Núria Bonet; Marc Garcia-Garcerà; Angela Domínguez; Fernando González-Candelas; Francesc Calafell Journal: PLoS One Date: 2015-09-17 Impact factor: 3.240
Authors: Peter S Askovich; Catherine J Sanders; Carrie M Rosenberger; Alan H Diercks; Pradyot Dash; Garnet Navarro; Peter Vogel; Peter C Doherty; Paul G Thomas; Alan Aderem Journal: PLoS One Date: 2013-09-20 Impact factor: 3.240
Authors: Ariel Rodriguez; Ana Falcon; Maria Teresa Cuevas; Francisco Pozo; Susana Guerra; Blanca García-Barreno; Pamela Martinez-Orellana; Pilar Pérez-Breña; Maria Montoya; Jose Antonio Melero; Manuel Pizarro; Juan Ortin; Inmaculada Casas; Amelia Nieto Journal: PLoS One Date: 2013-01-10 Impact factor: 3.240
Authors: Román Alejandro García-Ramírez; Alejandra Ramírez-Venegas; Roger Quintana-Carrillo; Ángel Eduardo Camarena; Ramcés Falfán-Valencia; Juan Manuel Mejía-Aranguré Journal: PLoS One Date: 2015-12-14 Impact factor: 3.240