Y-H Kuan1, Y-C Li, F-M Huang, Y-C Chang. 1. Department of Pharmacology, Chung Shan Medical University, 110 Sec. 1 Chien-Kuo N. Road, Taichung, Taiwan.
Abstract
AIM: To evaluate the expression of tumour necrosis factor-α and surface antigens by bisphenol A-glycidyl-methacrylate (BisGMA) on murine macrophage cell line RAW264.7. METHODOLOGY: Cytotoxicity was measured by tetrazolium bromide reduction assay. Tumour necrosis factor (TNF)-α was analysed by enzyme-linked immunosorbent assay. Cell surface antigens were investigated by flowcytometry. Statistical analyses were performed using anova followed by the Bonferroni's t-test for multigroup comparisons. RESULTS: BisGMA exhibited cytotoxicity to RAW264.7 in a dose-dependent manner (P < 0.05) during 2-h incubation period. BisGMA was found to increase TNF-α secretion in a dose-dependent manner (P < 0.05). In addition, CD11, CD14, CD45, CD54, CD40, CD80, and MHC II were significantly stimulated by BisGMA in a dose-dependent manner (P < 0.05). However, MHC I expression was not affected by BisGMA (P > 0.05). CONCLUSIONS: Taken together, the ability of macrophages to induce an appropriate immune response when exposed to BisGMA has the potential to upregulate TNF-α production and expression of surface antigens.
AIM: To evaluate the expression of tumour necrosis factor-α and surface antigens by bisphenol A-glycidyl-methacrylate (BisGMA) on murine macrophage cell line RAW264.7. METHODOLOGY:Cytotoxicity was measured by tetrazolium bromide reduction assay. Tumour necrosis factor (TNF)-α was analysed by enzyme-linked immunosorbent assay. Cell surface antigens were investigated by flowcytometry. Statistical analyses were performed using anova followed by the Bonferroni's t-test for multigroup comparisons. RESULTS:BisGMA exhibited cytotoxicity to RAW264.7 in a dose-dependent manner (P < 0.05) during 2-h incubation period. BisGMA was found to increase TNF-α secretion in a dose-dependent manner (P < 0.05). In addition, CD11, CD14, CD45, CD54, CD40, CD80, and MHC II were significantly stimulated by BisGMA in a dose-dependent manner (P < 0.05). However, MHC I expression was not affected by BisGMA (P > 0.05). CONCLUSIONS: Taken together, the ability of macrophages to induce an appropriate immune response when exposed to BisGMA has the potential to upregulate TNF-α production and expression of surface antigens.
Authors: Jinpeng Li; Anthony Bach; Robert B Crawford; Ashwini S Phadnis-Moghe; Weimin Chen; Shawna D'Ingillo; Natalia Kovalova; Jose E Suarez-Martinez; Jiajun Zhou; Barbara L F Kaplan; Norbert E Kaminski Journal: Toxicology Date: 2018-02-07 Impact factor: 4.221
Authors: N Couleau; J Falla; A Beillerot; E Battaglia; M D'Innocenzo; S Plançon; P Laval-Gilly; A Bennasroune Journal: PLoS One Date: 2015-07-02 Impact factor: 3.240
Authors: Han-Seok Choi; Soon Re Kim; Se Hyang Hong; Jin Mo Ku; Min Kyoung Kim; Hye Sook Seo; Sung-Gook Cho; Sangtae Shin; Yong Cheol Shin; Seong-Gyu Ko Journal: Evid Based Complement Alternat Med Date: 2013-06-09 Impact factor: 2.629