Literature DB >> 2226497

Evaluation of a line immunoassay for the differential detection of antibodies to human immunodeficiency virus.

R Vranckx1.   

Abstract

A newly introduced single strip line immunoassay was evaluated as a possible alternative to Western blot for the differential detection of antibodies to multiple HIV-1 and HIV-2 antigens in human serum. In a panel of 153 sera found to be positive twice in currently used screening tests, the line immunoassay and the Western blot detected 44 true positive results. Using Western blot as reference method, the line immunoassay gave 100% specificity and sensitivity. These results suggest that the line immunoassay may be a viable alternative to Western blot.

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Year:  1990        PMID： 2226497     DOI： 10.1007/bf01964271

Source DB:  PubMed          Journal:  Eur J Clin Microbiol Infect Dis        ISSN： 0934-9723            Impact factor:   3.267


  7 in total

Review 1.  Report of the Third Consensus Conference on HIV testing sponsored by the Association of State and Territorial Public Health Laboratory Directors.

Authors:  W J Hausler
Journal:  Infect Control Hosp Epidemiol       Date:  1988-08       Impact factor: 3.254

2.  HIV-antibody testing and false-positive p24 reactivity.

Authors:  B Kvinesdal; E Lauritzen; A G Poulsen; B F Vestergaard
Journal:  Vox Sang       Date:  1989       Impact factor: 2.144

Review 3.  Chemical synthesis of peptides and proteins.

Authors:  A Marglin; R B Merrifield
Journal:  Annu Rev Biochem       Date:  1970       Impact factor: 23.643

4.  Blood donors with indeterminate anti-p24gag reactivity in HIV-1 western blot: absence of infectivity to transfused patients and in virus culture.

Authors:  C L van der Poel; P N Lelie; H W Reesink; P J van Exel-Oehlers; M Tersmette; R van den Akker; M Gonzalves; J G Huisman
Journal:  Vox Sang       Date:  1989       Impact factor: 2.144

5.  Comparison of Western blot (immunoblot) based on recombinant-derived p41 with conventional tests for serodiagnosis of human immunodeficiency virus infections.

Authors:  J M Hofbauer; T F Schulz; P Hengster; C Larcher; R Zangerle; H Kofler; P Fritsch; H Wachter; M P Dierich
Journal:  J Clin Microbiol       Date:  1988-01       Impact factor: 5.948

6.  Measurement of the false positive rate in a screening program for human immunodeficiency virus infections.

Authors:  D S Burke; J F Brundage; R R Redfield; J J Damato; C A Schable; P Putman; R Visintine; H I Kim
Journal:  N Engl J Med       Date:  1988-10-13       Impact factor: 91.245

7.  Specificities and sensitivities of three systems for determination of antibodies to human immunodeficiency virus by electrophoretic immunoblotting.

Authors:  J Blomberg; P J Klasse
Journal:  J Clin Microbiol       Date:  1988-01       Impact factor: 5.948
  7 in total
  2 in total

1.  Field evaluation of a combination of monospecific enzyme-linked immunosorbent assays for type-specific diagnosis of human immunodeficiency virus type 1 (HIV-1) and HIV-2 infections in HIV-seropositive persons in Abidjan, Ivory Coast.

Authors:  J N Nkengasong; C Maurice; S Koblavi; M Kalou; C Bile; D Yavo; E Boateng; S Z Wiktor; A E Greenberg
Journal:  J Clin Microbiol       Date:  1998-01       Impact factor: 5.948

2.  Evaluation of a line immunoassay for simultaneous confirmation of antibodies to HIV-1 and HIV-2.

Authors:  K Fransen; D E Pollet; M Peeters; I van Kerckhoven; G Beelaert; G Vercauteren; P Piot; G van der Groen
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1991-11       Impact factor: 3.267
  2 in total

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