Literature DB >> 22264532

Measuring membrane protein dynamics in neurons using fluorescence recovery after photobleach.

Inmaculada M González-González1, Frédéric Jaskolski, Yves Goldberg, Michael C Ashby, Jeremy M Henley.   

Abstract

The use of genetically encoded fluorescent tags such as green fluorescent protein (GFP) as reporters to monitor processes in living cells has transformed cell biology. One major application for these tools has been to analyze protein dynamics in neurons. In particular, fluorescence recovery after photobleach (FRAP) of surface expressed fluorophore-tagged proteins has been instrumental to addressing outstanding questions about how neurons orchestrate the synaptic delivery of proteins. Here, we provide an overview of the methodology, equipment, and analysis required to perform, analyze, and interpret these experiments.
Copyright © 2012 Elsevier Inc. All rights reserved.

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Year:  2012        PMID： 22264532      PMCID： PMC3314508          DOI： 10.1016/B978-0-12-391857-4.00006-9

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  25 in total

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3.  Mobility measurement by analysis of fluorescence photobleaching recovery kinetics.

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Review 9.  It's green outside: tracking cell surface proteins with pH-sensitive GFP.

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Review 10.  Synaptic receptor trafficking: the lateral point of view.

Authors:  F Jaskolski; J M Henley
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  8 in total

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7.  Postsynaptic kainate receptor recycling and surface expression are regulated by metabotropic autoreceptor signalling.

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  8 in total

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