INTRODUCTION: The presence of microorganisms in gastric fluid in neonates at birth is postulated to reflect antenatal infection and also to be associated with the development of bronchopulmonary dysplasia (BPD). RESULTS: A logistic regression analysis, after controlling for other risk factors, indicated that Ureaplasma-positive infants were not at increased risk for moderate/severe BPD (adjusted odds ratio (OR): 2.58, 95% confidence interval (CI): 0.57-6.89, P = 0.12). However, the association between the presence of Ureaplasma species and the risk for moderate/severe BPD increased significantly in infants on mechanical ventilation (MV) ≥2 wk (adjusted OR: 4.17, 95% CI: 1.62-44.1, P = 0.009). An analysis using a lung injury marker indicated that Ureaplasma-positive infants with MV ≥2 wk, but not other infants, showed higher serum KL-6 levels in samples taken from cord blood, and that KL-6 levels increased time-dependently up to 4 wk of age. DISCUSSION: Antenatal exposure to Ureaplasma species induces lung injury prior to birth and synergistically contributes to the development of BPD in infants requiring prolonged MV (≥2 wk). METHODS: We recovered gastric fluid specimens from 122 infants with gestational age (GA) <29 wk or birth weight <1,000 g to investigate whether these microorganisms influence respiratory outcome of BPD. A PCR analysis was used to detect urease and 16S ribosomal RNA (rRNA) genes to classify neonates into Ureaplasma-positive or Ureaplasma-negative infants.
INTRODUCTION: The presence of microorganisms in gastric fluid in neonates at birth is postulated to reflect antenatal infection and also to be associated with the development of bronchopulmonary dysplasia (BPD). RESULTS: A logistic regression analysis, after controlling for other risk factors, indicated that Ureaplasma-positive infants were not at increased risk for moderate/severe BPD (adjusted odds ratio (OR): 2.58, 95% confidence interval (CI): 0.57-6.89, P = 0.12). However, the association between the presence of Ureaplasma species and the risk for moderate/severe BPD increased significantly in infants on mechanical ventilation (MV) ≥2 wk (adjusted OR: 4.17, 95% CI: 1.62-44.1, P = 0.009). An analysis using a lung injury marker indicated that Ureaplasma-positive infants with MV ≥2 wk, but not other infants, showed higher serum KL-6 levels in samples taken from cord blood, and that KL-6 levels increased time-dependently up to 4 wk of age. DISCUSSION: Antenatal exposure to Ureaplasma species induces lung injury prior to birth and synergistically contributes to the development of BPD in infants requiring prolonged MV (≥2 wk). METHODS: We recovered gastric fluid specimens from 122 infants with gestational age (GA) <29 wk or birth weight <1,000 g to investigate whether these microorganisms influence respiratory outcome of BPD. A PCR analysis was used to detect urease and 16S ribosomal RNA (rRNA) genes to classify neonates into Ureaplasma-positive or Ureaplasma-negative infants.
Authors: Kirsten Glaser; Christine Silwedel; Markus Fehrholz; Ana M Waaga-Gasser; Birgit Henrich; Heike Claus; Christian P Speer Journal: Front Cell Infect Microbiol Date: 2017-11-28 Impact factor: 5.293
Authors: Kenichiro Motomura; Roberto Romero; Yi Xu; Kevin R Theis; Jose Galaz; Andrew D Winters; Rebecca Slutsky; Valeria Garcia-Flores; Chengrui Zou; Dustyn Levenson; Robert Para; Madison M Ahmad; Derek Miller; Chaur-Dong Hsu; Nardhy Gomez-Lopez Journal: mBio Date: 2020-06-23 Impact factor: 7.867
Authors: Kirsten Glaser; Anna Gradzka-Luczewska; Marta Szymankiewicz-Breborowicz; Natalia Kawczynska-Leda; Birgit Henrich; Ana Maria Waaga-Gasser; Christian P Speer Journal: Front Cell Infect Microbiol Date: 2019-04-02 Impact factor: 5.293
Authors: Jasper V Been; Sizzle F Vanterpool; Jasmijn D E de Rooij; G Ingrid J G Rours; René F Kornelisse; Martien C J M van Dongen; Christel J A W van Gool; Ronald R de Krijger; Peter Andriessen; Luc J I Zimmermann; Boris W Kramer Journal: PLoS One Date: 2012-10-05 Impact factor: 3.240