Literature DB >> 22252630

Glycosaminoglycan chain analysis and characterization (glycosylation/epimerization).

Shuji Mizumoto1, Kazuyuki Sugahara.   

Abstract

Glycosaminoglycans (GAGs) including chondroitin sulfate (CS), dermatan sulfate (DS), and heparan sulfate/heparin (HS/Hep) are linear polysaccharides and involved in the regulation of various biological events through interaction with functional proteins. GAGs are modified by sulfation at various positions of each saccharide residue and the epimerization of uronic acid residues during the chain's biosynthesis, resulting in enormous structural diversity. This structural diversity is the basis for the wide range of biological activities of GAGs. Thus, the structural analysis of GAGs is key to understanding their biological functions. This chapter describes detailed instructions for the extraction and structural analysis of GAGs from cultured cells and tissues using a combination of GAG-degrading enzymes and high-performance liquid chromatography.

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Year:  2012        PMID： 22252630     DOI： 10.1007/978-1-61779-498-8_7

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  15 in total

1.  Hyaluronidase-4 is produced by mast cells and can cleave serglycin chondroitin sulfate chains into lower molecular weight forms.

Authors:  Brooke L Farrugia; Shuji Mizumoto; Megan S Lord; Robert L O'Grady; Rhiannon P Kuchel; Shuhei Yamada; John M Whitelock
Journal:  J Biol Chem       Date:  2019-06-07       Impact factor: 5.157

2.  Analysis of glycosaminoglycan-derived, precolumn, 2-aminoacridone-labeled disaccharides with LC-fluorescence and LC-MS detection.

Authors:  Nicola Volpi; Fabio Galeotti; Bo Yang; Robert J Linhardt
Journal:  Nat Protoc       Date:  2014-02-06       Impact factor: 13.491

3.  Vascular abnormalities in the placenta of Chst14-/- fetuses: implications in the pathophysiology of perinatal lethality of the murine model and vascular lesions in human CHST14/D4ST1 deficiency.

Authors:  Takahiro Yoshizawa; Shuji Mizumoto; Yuki Takahashi; Shin Shimada; Kazuyuki Sugahara; Jun Nakayama; Shin'ichi Takeda; Yoshihiro Nomura; Yuko Nitahara-Kasahara; Takashi Okada; Kiyoshi Matsumoto; Shuhei Yamada; Tomoki Kosho
Journal:  Glycobiology       Date:  2018-02-01       Impact factor: 4.313

4.  Skeletal dysplasia in a consanguineous clan from the island of Nias/Indonesia is caused by a novel mutation in B3GAT3.

Authors:  Birgit S Budde; Shuji Mizumoto; Ryo Kogawa; Christian Becker; Janine Altmüller; Holger Thiele; Franz Rüschendorf; Mohammad R Toliat; Gerrit Kaleschke; Johannes M Hämmerle; Wolfgang Höhne; Kazuyuki Sugahara; Peter Nürnberg; Ingo Kennerknecht
Journal:  Hum Genet       Date:  2015-04-19       Impact factor: 4.132

5.  Interaction of chondroitin sulfate and dermatan sulfate from various biological sources with heparin-binding growth factors and cytokines.

Authors:  Shuji Mizumoto; Duriya Fongmoon; Kazuyuki Sugahara
Journal:  Glycoconj J       Date:  2012-12-29       Impact factor: 2.916

6.  Compositional analysis of the glycosaminoglycan family in velvet antlers of Sika deer (Cervus nippon) at different growing stages.

Authors:  Naoko Takeda-Okuda; Shuji Mizumoto; Zui Zhang; Soo-Ki Kim; Chi-Ho Lee; Byong-Tae Jeon; Yoshinao Z Hosaka; Kenji Kadomatsu; Shuhei Yamada; Jun-Ichi Tamura
Journal:  Glycoconj J       Date:  2019-01-24       Impact factor: 2.916

7.  Mutations in B3GALT6, which encodes a glycosaminoglycan linker region enzyme, cause a spectrum of skeletal and connective tissue disorders.

Authors:  Masahiro Nakajima; Shuji Mizumoto; Noriko Miyake; Ryo Kogawa; Aritoshi Iida; Hironori Ito; Hiroshi Kitoh; Aya Hirayama; Hiroshi Mitsubuchi; Osamu Miyazaki; Rika Kosaki; Reiko Horikawa; Angeline Lai; Roberto Mendoza-Londono; Lucie Dupuis; David Chitayat; Andrew Howard; Gabriela F Leal; Denise Cavalcanti; Yoshinori Tsurusaki; Hirotomo Saitsu; Shigehiko Watanabe; Ekkehart Lausch; Sheila Unger; Luisa Bonafé; Hirofumi Ohashi; Andrea Superti-Furga; Naomichi Matsumoto; Kazuyuki Sugahara; Gen Nishimura; Shiro Ikegawa
Journal:  Am J Hum Genet       Date:  2013-05-09       Impact factor: 11.025

8.  Receptor protein tyrosine phosphatase σ binds to neurons in the adult mouse brain.

Authors:  Jae-Hyuk Yi; Yasuhiro Katagiri; Panpan Yu; Jacob Lourie; Nathanael J Bangayan; Aviva J Symes; Herbert M Geller
Journal:  Exp Neurol       Date:  2014-02-14       Impact factor: 5.330

9.  Expression of N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase involved in chondroitin sulfate synthesis is responsible for pulmonary metastasis.

Authors:  Shuji Mizumoto; Moto Watanabe; Shuhei Yamada; Kazuyuki Sugahara
Journal:  Biomed Res Int       Date:  2013-02-20       Impact factor: 3.411

Review 10.  Biological functions of iduronic acid in chondroitin/dermatan sulfate.

Authors:  Martin A Thelin; Barbara Bartolini; Jakob Axelsson; Renata Gustafsson; Emil Tykesson; Edgar Pera; Åke Oldberg; Marco Maccarana; Anders Malmstrom
Journal:  FEBS J       Date:  2013-03-28       Impact factor: 5.542
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