Literature DB >> 22251424

Dead-end hollow-fiber ultrafiltration for concentration and enumeration of Escherichia coli and broad-host-range plasmid DNA from wastewater.

Kyle L Asfahl1, Mary C Savin.   

Abstract

Broad-host-range plasmids can facilitate dissemination of antibiotic resistance determinants among diverse bacterial populations. We evaluated hollow-fiber ultrafiltration for increases in detection efficiency of broad-host-range plasmids and Escherichia coli DNA in wastewater. Ultrafiltration followed by PCR showed limited increases in DNA detection and quantification in effluent compared with membrane filtration alone. Copyright Â
© 2012 Elsevier B.V. All rights reserved.

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Year:  2012        PMID： 22251424      PMCID： PMC3289581          DOI： 10.1016/j.mimet.2012.01.005

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  17 in total

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4.  Broad-host-range plasmids in treated wastewater effluent and receiving streams.

Authors:  Tatsuya Akiyama; Kyle L Asfahl; Mary C Savin
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Journal:  J Appl Microbiol       Date:  2009-02-16       Impact factor: 3.772

6.  Evaluation of ultrafiltration cartridges for a water sampling apparatus.

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7.  Detection and characterization of broad-host-range plasmids in environmental bacteria by PCR.

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Journal:  ISME J       Date:  2010-06-03       Impact factor: 10.302

Review 9.  Resistance to antibiotics: are we in the post-antibiotic era?

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10.  Development of a rapid method for simultaneous recovery of diverse microbes in drinking water by ultrafiltration with sodium polyphosphate and surfactants.

Authors:  Vincent R Hill; Amy L Polaczyk; Donghyun Hahn; Jothikumar Narayanan; Theresa L Cromeans; Jacquelin M Roberts; James E Amburgey
Journal:  Appl Environ Microbiol       Date:  2005-11       Impact factor: 4.792
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  1 in total

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