PURPOSE: To evaluate the impact of functional polymorphisms in genes related to DNA repair mechanisms (XRCC1, TP53, MSH2, MSH3, XPD), oxidative stress response (GSTP1, GSTA1, eNOS, SOD2) and fibroblast proliferation (TGFβ1) on the risk of acute skin toxicity in breast cancer patients receiving radiotherapy. MATERIAL AND METHODS: Skin toxicity was scored according to the Radiation Therapy Oncology Group criteria in 286 breast cancer patients who received radiotherapy after breast conserving surgery. Genotyping was conducted by PCR-RFLP analysis and real-time PCR allelic discrimination assay on genomic DNA extracted from peripheral blood. RESULTS: In the multivariate analysis, nominally significant associations, before multiple testing corrections, were found between XRCC1 T-77C (T carriers vs. CC, OR: 2.240, 95% CI: 1.015-4.941, P=0.046), eNOS G894T polymorphisms (TT vs. G carriers, OR: 2.473, 95% CI: 1.220-5.012, P=0.012), breast diameter (OR: 1.138, 95% CI: 1.001-1.293, P=0.048), boost dose-fractionation (3 Gy vs. no boost, OR: 4.902, 95% CI: 1.458-16.483, P=0.010) and ≥ grade 2 acute radiation skin toxicity in breast cancer patients. CONCLUSIONS: As our exploratory study suggests that XRCC1 T-77C and eNOS G874T may confer an increased risk of acute skin reactions to radiotherapy in breast cancer patients, further confirmatory studies are warranted to determine the clinical significance.
PURPOSE: To evaluate the impact of functional polymorphisms in genes related to DNA repair mechanisms (XRCC1, TP53, MSH2, MSH3, XPD), oxidative stress response (GSTP1, GSTA1, eNOS, SOD2) and fibroblast proliferation (TGFβ1) on the risk of acute skin toxicity in breast cancerpatients receiving radiotherapy. MATERIAL AND METHODS:Skin toxicity was scored according to the Radiation Therapy Oncology Group criteria in 286 breast cancerpatients who received radiotherapy after breast conserving surgery. Genotyping was conducted by PCR-RFLP analysis and real-time PCR allelic discrimination assay on genomic DNA extracted from peripheral blood. RESULTS: In the multivariate analysis, nominally significant associations, before multiple testing corrections, were found between XRCC1T-77C (T carriers vs. CC, OR: 2.240, 95% CI: 1.015-4.941, P=0.046), eNOSG894T polymorphisms (TT vs. G carriers, OR: 2.473, 95% CI: 1.220-5.012, P=0.012), breast diameter (OR: 1.138, 95% CI: 1.001-1.293, P=0.048), boost dose-fractionation (3 Gy vs. no boost, OR: 4.902, 95% CI: 1.458-16.483, P=0.010) and ≥ grade 2 acute radiation skin toxicity in breast cancerpatients. CONCLUSIONS: As our exploratory study suggests that XRCC1T-77C and eNOSG874T may confer an increased risk of acute skin reactions to radiotherapy in breast cancerpatients, further confirmatory studies are warranted to determine the clinical significance.
Authors: Asim Alam; Nitai D Mukhopadhyay; Yi Ning; Leonid B Reshko; Robert J G Cardnell; Omair Alam; Christopher S Rabender; Vasily A Yakovlev; Linda Walker; Mitchell S Anscher; Ross B Mikkelsen Journal: Int J Radiat Oncol Biol Phys Date: 2015-06-06 Impact factor: 7.038
Authors: K Yanar; U Çakatay; S Aydın; A Verim; P Atukeren; N E Özkan; K Karatoprak; T Cebe; S Turan; E Ozkök; G Korkmaz; C Cacına; O Küçükhüseyin; İ Yaylım Journal: Oxid Med Cell Longev Date: 2015-11-22 Impact factor: 6.543
Authors: Dae Sik Yang; Jung Ae Lee; Won Sup Yoon; Nam Kwon Lee; Young Je Park; Suk Lee; Chul Yong Kim; Gil Soo Son Journal: Biomed Res Int Date: 2016-08-07 Impact factor: 3.411