| Literature DB >> 22245333 |
Shuai Liu1, Pengfei Cai, Nan Hou, Xianyu Piao, Heng Wang, Tao Hung, Qijun Chen.
Abstract
Quantitative real-time polymerase chain reaction (qPCR), as one of the most sensitive and precise gene expression analysis methods, is frequently used to validate data obtained in high-through-put assays. qPCR requires reference genes with stable transcription for accurate normalization. However, no systematic studies on such genes have been performed in the genus Schistosoma japonicum. In this study, eight novel candidate genes selected from a microarray analysis and four commonly used reference genes were systematically validated in a series of qPCR experiments. Based on the results of geNorm, Normfinder, BestKeeper, and the comparative delta-cycle threshold (ΔCT) integrated analysis, the genes PSMD4, NDUFV2, and TPC2L were found to be most stably expressed in all S. japonicum developmental stages; meanwhile, ACTB and TUBA were found as the least stably expressed genes. This study provided, at the first time, data for genes that can be explored as reliable references in transcriptomic analysis of S. japonicum.Entities:
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Year: 2012 PMID： 22245333 DOI： 10.1016/j.molbiopara.2011.12.007
Source DB: PubMed Journal: Mol Biochem Parasitol ISSN： 0166-6851 Impact factor: 1.759