Literature DB >> 222332

Preparative isoelectric focussing of apolipoproteins C and E from human very low density lipoproteins.

Y L Marcel, M Bergseth, A C Nestruck.   

Abstract

The application of isoelectric focussing on a gel-stabilized layer for the separation of the Tris-urea-soluble apolipoproteins of very low density lipoproteins has been described. This method in one step, allows the separation of most apolipoproteins, which were then analyzed and characterized. Apolipoproteins CII and CI were isolated as single protein bands with apparent pI of 5.0 and 6.5, respectively. Apolipoprotein CII was biologically active and could activate lipoprotein lipase. Apolipoprotein CIII was separated into several protein bands with pI ranging from 4.7 to 5.1 as a function of their number of sialic acid residues. Apolipoprotein E was isolated and characterized into five polymorphic bands with pI of 5.7, 5.8, 5.9, 6.0, and 6.2, respectively.

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Year:  1979        PMID： 222332     DOI： 10.1016/0005-2760(79)90184-x

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  13 in total

1.  Purification, cloning and nucleotide sequence determination of cynomolgus monkey apolipoprotein C-II: comparison to the human sequence.

Authors:  B E Whitted; C K Castle; H G Polites; G W Melchior; K R Marotti
Journal:  Mol Cell Biochem       Date:  1989-10-05       Impact factor: 3.396

Review 2.  Apolipoprotein E.

Authors:  V I Zannis; J L Breslow
Journal:  Mol Cell Biochem       Date:  1982-01-16       Impact factor: 3.396

3.  Separation of the apoprotein components of human very low density lipoproteins by ion-paired, reversed-phase high performance liquid chromatography.

Authors:  W S Hancock; C A Bishop; A M Gotto; D R Harding; S M Lamplugh; J T Sparrow
Journal:  Lipids       Date:  1981-04       Impact factor: 1.880

4.  Lecithin:cholesterol acyltransferase-mediated modification of discoidal peripheral lymph high density lipoproteins: possible mechanism of formation of cholesterol-induced high density lipoproteins (HDLc) in cholesterol-fed dogs.

Authors:  L Dory; C H Sloop; L M Boquet; R L Hamilton; P S Roheim
Journal:  Proc Natl Acad Sci U S A       Date:  1983-06       Impact factor: 11.205

5.  Modulation of lipoprotein lipase activity by apolipoproteins. Effect of apolipoprotein C-III.

Authors:  C S Wang; W J McConathy; H U Kloer; P Alaupovic
Journal:  J Clin Invest       Date:  1985-02       Impact factor: 14.808

6.  Apolipoprotein C-II deficiency syndrome. Clinical features, lipoprotein characterization, lipase activity, and correction of hypertriglyceridemia after apolipoprotein C-II administration in two affected patients.

Authors:  G Baggio; E Manzato; C Gabelli; R Fellin; S Martini; G B Enzi; F Verlato; M R Baiocchi; D L Sprecher; M L Kashyap
Journal:  J Clin Invest       Date:  1986-02       Impact factor: 14.808

7.  Structure of apolipoprotein C-IIToronto, a nonfunctional human apolipoprotein.

Authors:  P W Connelly; G F Maguire; T Hofmann; J A Little
Journal:  Proc Natl Acad Sci U S A       Date:  1987-01       Impact factor: 11.205

8.  A rapid laser immunonephelometric assay for serum amyloid A (SAA) and its application to the diagnosis of kidney allograft rejection.

Authors:  G Hocke; H Ebel; K Bittner; T Müller; H Kaffarnik; A Steinmetz
Journal:  Klin Wochenschr       Date:  1989-04-17

9.  Characterization of monoclonal antibodies against human apolipoprotein E.

Authors:  R W Milne; P Douste-Blazy; Y L Marcel; L Retegui
Journal:  J Clin Invest       Date:  1981-07       Impact factor: 14.808

10.  Human apolipoprotein E isoprotein subclasses are genetically determined.

Authors:  V I Zannis; P W Just; J L Breslow
Journal:  Am J Hum Genet       Date:  1981-01       Impact factor: 11.025
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