BACKGROUND: The prognostic role of circulating tumor cells (CTCs) has been established for colorectal cancer (CRC). We investigated the qualitative and quantitative detection of CTC in the central (CVBC) and mesenteric (MVBC) venous blood compartments to elucidate the patterns of hematogenous tumor cell dissemination in patients with CRC. METHODS: A total of 200 patients were enrolled prospectively. Blood samples were collected from the tumor-draining vein and via a central venous line. CTCs were detected and quantified by using the CellSearch system. Factors associated with CTC detection in both compartments were analyzed by using univariate and multivariate analyses. RESULTS: CTC analyses were performed in the CVBC and MVBC in 200 and 80 patients, respectively. CTCs were found at a higher rate (P=0.01) and at a higher count (P=0.006) in the MVBC compared with the CVBC. On multivariate analyses, stage IV disease (odds ratio, 3.83; 95% confidence interval, 1.42-10.35) and increased preoperative carbohydrate antigen 19-9 level (odds ratio, 3.57; 1.30-9.79) were associated with CTC detection in the CVBC. CTCs were detected more frequently (P=0.05) and at higher numbers (P=0.05) in the CVBC of patients with low compared with mid or high rectal tumors. CONCLUSIONS: The qualitative and quantitative detection of CTCs is higher in the MVBC compared with the CVBC of patients with CRC.
BACKGROUND: The prognostic role of circulating tumor cells (CTCs) has been established for colorectal cancer (CRC). We investigated the qualitative and quantitative detection of CTC in the central (CVBC) and mesenteric (MVBC) venous blood compartments to elucidate the patterns of hematogenous tumor cell dissemination in patients with CRC. METHODS: A total of 200 patients were enrolled prospectively. Blood samples were collected from the tumor-draining vein and via a central venous line. CTCs were detected and quantified by using the CellSearch system. Factors associated with CTC detection in both compartments were analyzed by using univariate and multivariate analyses. RESULTS: CTC analyses were performed in the CVBC and MVBC in 200 and 80 patients, respectively. CTCs were found at a higher rate (P=0.01) and at a higher count (P=0.006) in the MVBC compared with the CVBC. On multivariate analyses, stage IV disease (odds ratio, 3.83; 95% confidence interval, 1.42-10.35) and increased preoperative carbohydrate antigen 19-9 level (odds ratio, 3.57; 1.30-9.79) were associated with CTC detection in the CVBC. CTCs were detected more frequently (P=0.05) and at higher numbers (P=0.05) in the CVBC of patients with low compared with mid or high rectal tumors. CONCLUSIONS: The qualitative and quantitative detection of CTCs is higher in the MVBC compared with the CVBC of patients with CRC.
Authors: Vasudha Murlidhar; Rishindra M Reddy; Shamileh Fouladdel; Lili Zhao; Martin K Ishikawa; Svetlana Grabauskiene; Zhuo Zhang; Jules Lin; Andrew C Chang; Philip Carrott; William R Lynch; Mark B Orringer; Chandan Kumar-Sinha; Nallasivam Palanisamy; David G Beer; Max S Wicha; Nithya Ramnath; Ebrahim Azizi; Sunitha Nagrath Journal: Cancer Res Date: 2017-07-17 Impact factor: 12.701
Authors: Jussuf T Kaifi; Miriam Kunkel; David T Dicker; Jamal Joude; Joshua E Allen; Avisnata Das; Junjia Zhu; Zhaohai Yang; Nabeel E Sarwani; Guangfu Li; Kevin F Staveley-O'Carroll; Wafik S El-Deiry Journal: Cancer Biol Ther Date: 2015 Impact factor: 4.742
Authors: Jussuf T Kaifi; Miriam Kunkel; Junjia Zhu; David T Dicker; Niraj Gusani; Zhaohai Yang; Nabeel E Sarwani; Guangfu Li; Eric T Kimchi; Kevin F Staveley-O'Carroll; Wafik S El-Deiry Journal: Cancer Biol Ther Date: 2013-10-23 Impact factor: 4.742
Authors: Ulrich Bork; Robert Grützmann; Nuh N Rahbari; Sebastian Schölch; Marius Distler; Christoph Reissfelder; Moritz Koch; Jürgen Weitz Journal: World J Gastroenterol Date: 2014-08-14 Impact factor: 5.742
Authors: Maria Gazouli; Anna Lyberopoulou; Pericles Pericleous; Spyros Rizos; Gerassimos Aravantinos; Nikolaos Nikiteas; Nicholas P Anagnou; Efstathios P Efstathopoulos Journal: World J Gastroenterol Date: 2012-08-28 Impact factor: 5.742
Authors: Johannes C Fischer; Dieter Niederacher; Stefan A Topp; Ellen Honisch; Sarah Schumacher; Norma Schmitz; Luisa Zacarias Föhrding; Christian Vay; Imke Hoffmann; Nikola S Kasprowicz; Philip G Hepp; Svjetlana Mohrmann; Ulrike Nitz; Antje Stresemann; Thomas Krahn; Tanja Henze; Evelyn Griebsch; Katharina Raba; Jutta M Rox; Folker Wenzel; Christoph Sproll; Wolfgang Janni; Tanja Fehm; Christoph A Klein; Wolfram Trudo Knoefel; Nikolas H Stoecklein Journal: Proc Natl Acad Sci U S A Date: 2013-09-24 Impact factor: 11.205