Victor Vitvitsky1, Omer Kabil, Ruma Banerjee. 1. Biological Chemistry Department, University of Michigan Medical Center, Ann Arbor, Michigan 48109-0600, USA.
Abstract
AIMS: Hydrogen sulfide (H(2)S) is a signaling molecule, which influences many physiological processes. While H(2)S is produced and degraded in many cell types, the kinetics of its turnover in different tissues has not been reported. In this study, we have assessed the rates of H(2)S production in murine liver, kidney, and brain homogenates at pH 7.4, 37°C, and at physiologically relevant cysteine concentrations. We have also studied the kinetics of H(2)S clearance by liver, kidney, and brain homogenates under aerobic and anaerobic conditions. RESULTS: We find that the rate of H(2)S production by these tissue homogenates is considerably higher than background rates observed in the absence of exogenous substrates. An exponential decay of H(2)S with time is observed and, as expected, is significantly faster under aerobic conditions. The half-life for H(2)S under aerobic conditions is 2.0, 2.8, and 10.0 min with liver, kidney, and brain homogenate, respectively. Western-blot analysis of the sulfur dioxygenase, ETHE1, involved in H(2)S catabolism, demonstrates higher steady-state protein levels in liver and kidney versus brain. INNOVATION: By combining experimental and simulation approaches, we demonstrate high rates of tissue H(2)S turnover and provide estimates of steady-state H(2)S levels. CONCLUSION: Our study reveals that tissues maintain a high metabolic flux of sulfur through H(2)S, providing a rationale for how H(2)S levels can be rapidly regulated.
AIMS: Hydrogen sulfide (H(2)S) is a signaling molecule, which influences many physiological processes. While H(2)S is produced and degraded in many cell types, the kinetics of its turnover in different tissues has not been reported. In this study, we have assessed the rates of H(2)S production in murine liver, kidney, and brain homogenates at pH 7.4, 37°C, and at physiologically relevant cysteine concentrations. We have also studied the kinetics of H(2)S clearance by liver, kidney, and brain homogenates under aerobic and anaerobic conditions. RESULTS: We find that the rate of H(2)S production by these tissue homogenates is considerably higher than background rates observed in the absence of exogenous substrates. An exponential decay of H(2)S with time is observed and, as expected, is significantly faster under aerobic conditions. The half-life for H(2)S under aerobic conditions is 2.0, 2.8, and 10.0 min with liver, kidney, and brain homogenate, respectively. Western-blot analysis of the sulfur dioxygenase, ETHE1, involved in H(2)S catabolism, demonstrates higher steady-state protein levels in liver and kidney versus brain. INNOVATION: By combining experimental and simulation approaches, we demonstrate high rates of tissue H(2)S turnover and provide estimates of steady-state H(2)S levels. CONCLUSION: Our study reveals that tissues maintain a high metabolic flux of sulfur through H(2)S, providing a rationale for how H(2)S levels can be rapidly regulated.
Authors: Victor Vitvitsky; Sanjana Dayal; Sally Stabler; You Zhou; Hong Wang; Steven R Lentz; Ruma Banerjee Journal: Am J Physiol Regul Integr Comp Physiol Date: 2004-03-11 Impact factor: 3.619
Authors: Fabian H Müller; Tiago M Bandeiras; Tim Urich; Miguel Teixeira; Cláudio M Gomes; Arnulf Kletzin Journal: Mol Microbiol Date: 2004-08 Impact factor: 3.501
Authors: Ricardo de Pascual; Andrés M Baraibar; Iago Méndez-López; Martín Pérez-Ciria; Ignacio Polo-Vaquero; Luis Gandía; Sunny E Ohia; Antonio G García; Antonio M G de Diego Journal: Pflugers Arch Date: 2018-05-02 Impact factor: 3.657