Literature DB >> 2222483

Extracellular depolymerization of hyaluronic acid in cultured human skin fibroblasts.

T Nakamura1, K Takagaki, K Kubo, A Morikawa, S Tamura, M Endo.   

Abstract

The chain length of [3H]hyaluronic acid synthesized by cultivating human skin fibroblasts in the presence of [3H]glucosamine was investigated. [3H]Hyaluronic acid obtained from the matrix fraction was excluded from a Sepharose CL-2B column irrespective of the incubation period, whereas that from the medium was depolymerized into a constant chain length (Mr = 40,000). The reducing and non-reducing terminals of the depolymerized hyaluronic acid were N-acetylglucosamine and glucuronic acid, respectively. Prolonged incubation produced no oligosaccharides as shown by examination of hyaluronidase digests, suggesting the presence of a novel endo-beta-N-acetylglucosaminidase in cultured human skin fibroblasts.

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Year:  1990        PMID： 2222483     DOI： 10.1016/s0006-291x(05)80174-3

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  6 in total

1.  Depolymerization of hyaluronan by sonication.

Authors:  K Kubo; T Nakamura; K Takagaki; Y Yoshida; M Endo
Journal:  Glycoconj J       Date:  1993-12       Impact factor: 2.916

2.  Agarose and polyacrylamide gel electrophoresis methods for molecular mass analysis of 5- to 500-kDa hyaluronan.

Authors:  Shardul Bhilocha; Ripal Amin; Monika Pandya; Han Yuan; Mihir Tank; Jaclyn LoBello; Anastasia Shytuhina; Wenlan Wang; Hans-Georg Wisniewski; Carol de la Motte; Mary K Cowman
Journal:  Anal Biochem       Date:  2011-05-27       Impact factor: 3.365

3.  Cytokine regulation of human lung fibroblast hyaluronan (hyaluronic acid) production. Evidence for cytokine-regulated hyaluronan (hyaluronic acid) degradation and human lung fibroblast-derived hyaluronidase.

Authors:  P M Sampson; C L Rochester; B Freundlich; J A Elias
Journal:  J Clin Invest       Date:  1992-10       Impact factor: 14.808

4.  KIAA1199, a deafness gene of unknown function, is a new hyaluronan binding protein involved in hyaluronan depolymerization.

Authors:  Hiroyuki Yoshida; Aya Nagaoka; Ayumi Kusaka-Kikushima; Megumi Tobiishi; Keigo Kawabata; Tetsuya Sayo; Shingo Sakai; Yoshinori Sugiyama; Hiroyuki Enomoto; Yasunori Okada; Shintaro Inoue
Journal:  Proc Natl Acad Sci U S A       Date:  2013-03-18       Impact factor: 11.205

5.  Secreted KIAA1199 promotes the progression of rheumatoid arthritis by mediating hyaluronic acid degradation in an ANXA1-dependent manner.

Authors:  Wei Zhang; Guoyu Yin; Heping Zhao; Hanzhi Ling; Zhen Xie; Chipeng Xiao; Yan Chen; Yufan Lin; Tao Jiang; Shengwei Jin; Jianguang Wang; Xinyu Yang
Journal:  Cell Death Dis       Date:  2021-01-20       Impact factor: 8.469

6.  Hyaluronan-binding protein in endothelial cell morphogenesis.

Authors:  S D Banerjee; B P Toole
Journal:  J Cell Biol       Date:  1992-11       Impact factor: 10.539
  6 in total

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