Literature DB >> 2222473

Efflux of bis-carboxyethyl-carboxyfluorescein (BCECF) by a novel ATP-dependent transport mechanism in epithelial cells.

C N Allen1, E S Harpur, T J Gray, N L Simmons, B H Hirst.   

Abstract

The efflux of the intracellular pH fluorochrome 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) was quantified in four cultured epithelial cell lines; HCT-8, T84, HGT-1 and MDCK. BCECF efflux was time-dependent, and after 5 h 45-91% of the initial BCECF loaded was extracellular, efflux being greatest in MDCK cells. Depletion of cellular ATP approximately halved BCECF efflux. BCECF efflux was inhibited by indomethacin, vinblastine and verapamil, but not by nifedipine or reserpine. Certain features of BCECF efflux resemble drug efflux in multidrug resistant cells, but inhibition of efflux displays a distinct pharmacological profile suggesting BCECF is a substrate for a novel ATP-dependent transport system.

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Year:  1990        PMID： 2222473     DOI： 10.1016/s0006-291x(05)80203-7

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  8 in total

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Authors:  D Molenaar; H Bolhuis; T Abee; B Poolman; W N Konings
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2.  Intestinal elimination of ofloxacin enantiomers in the rat: evidence of a carrier-mediated process.

Authors:  L Rabbaa; S Dautrey; N Colas-Linhart; C Carbon; R Farinotti
Journal:  Antimicrob Agents Chemother       Date:  1996-09       Impact factor: 5.191

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Journal:  J Comp Physiol B       Date:  2004-10-20       Impact factor: 2.200

4.  Carrier mechanisms involved in the transepithelial transport of bis(POM)-PMEA and its metabolites across Caco-2 monolayers.

Authors:  P Annaert; J Van Gelder; L Naesens; E De Clercq; G Van den Mooter; R Kinget; P Augustijns
Journal:  Pharm Res       Date:  1998-08       Impact factor: 4.200

5.  A Novel Method for Continuous Determination of the Intracellular pH in Bacteria with the Internally Conjugated Fluorescent Probe 5 (and 6-)-Carboxyfluorescein Succinimidyl Ester.

Authors:  P Breeuwer; J Drocourt; F M Rombouts; T Abee
Journal:  Appl Environ Microbiol       Date:  1996-01       Impact factor: 4.792

6.  Intracellular pH in renal tubules in situ: single-cell measurements by confocal laserscan microscopy.

Authors:  M Weinlich; G Capasso; R K Kinne
Journal:  Pflugers Arch       Date:  1993-02       Impact factor: 3.657

7.  Energy-dependent, carrier-mediated extrusion of carboxyfluorescein from Saccharomyces cerevisiae allows rapid assessment of cell viability by flow cytometry.

Authors:  P Breeuwer; J L Drocourt; F M Rombouts; T Abee
Journal:  Appl Environ Microbiol       Date:  1994-05       Impact factor: 4.792

8.  Epithelial secretion of vinblastine by human intestinal adenocarcinoma cell (HCT-8 and T84) layers expressing P-glycoprotein.

Authors:  J Hunter; B H Hirst; N L Simmons
Journal:  Br J Cancer       Date:  1991-09       Impact factor: 7.640
  8 in total

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