| Literature DB >> 22223621 |
Hsien-Po Chiu1, Jan Grünewald, Xueshi Hao, Ansgar Brock, Linda Okach, Tetsuo Uno, Bernhard H Geierstanger.
Abstract
Sticky residue: Pyrroline-carboxy-lysine (Pcl) can be readily incorporated into proteins expressed in E. coli and mammalian cells by using the pyrrolysyl tRNA/tRNA synthetase pair. Pcl can be used as a single amino acid purification tag and can be site-specifically modified with functional probes during the elution process.Entities:
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Year: 2012 PMID： 22223621 DOI： 10.1002/cbic.201100684
Source DB: PubMed Journal: Chembiochem ISSN： 1439-4227 Impact factor: 3.164