Literature DB >> 22222530

Isolation and characterization of embryonic and adult epicardium and epicardium-derived cells.

Bin Zhou1, William T Pu.   

Abstract

Epicardium is the outer cell layer of the heart. Its integrity and function are essential for normal heart development. To study the role of epicardium in both fetal and adult hearts, it is desirable to isolate and culture pure populations of these cells. Here we describe methods with Cre-loxP technology to lineage tag epicardial cells (EpiCs) and epicardium-derived cells (EPDCs), dissociate and isolate them by flow-activated cytometry sorting (FACS), and characterize them by quantitative PCR and immunostaining. This platform allows further characterization and manipulation of EpiCs and EPDCs for expression studies and functional assays.

Entities:  

Mesh：

Year:  2012        PMID： 22222530     DOI： 10.1007/978-1-61779-523-7_15

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  12 in total

Review 1.  Thymosin β4 and cardiac regeneration: are we missing a beat?

Authors:  David C Gajzer; Jerome Balbin; Hina W Chaudhry
Journal:  Stem Cell Rev Rep       Date:  2013-06       Impact factor: 5.739

2.  Epicardial Outgrowth Culture Assay and Ex Vivo Assessment of Epicardial-derived Cell Migration.

Authors:  Michael A Trembley; Lissette S Velasquez; Eric M Small
Journal:  J Vis Exp       Date:  2016-03-18       Impact factor: 1.355

3.  Human Pluripotent Stem Cell-Derived Multipotent Vascular Progenitors of the Mesothelium Lineage Have Utility in Tissue Engineering and Repair.

Authors:  Thomas Colunga; Miranda Hayworth; Sebastian Kreß; David M Reynolds; Luoman Chen; Kristopher L Nazor; Johannes Baur; Amar M Singh; Jeanne F Loring; Marco Metzger; Stephen Dalton
Journal:  Cell Rep       Date:  2019-03-05       Impact factor: 9.423

4.  Single epicardial cell transcriptome sequencing identifies Caveolin 1 as an essential factor in zebrafish heart regeneration.

Authors:  Jingli Cao; Adam Navis; Ben D Cox; Amy L Dickson; Matthew Gemberling; Ravi Karra; Michel Bagnat; Kenneth D Poss
Journal:  Development       Date:  2015-12-10       Impact factor: 6.868

5.  Isolation and culture of mouse proepicardium using serum-free conditions.

Authors:  Robert J Garriock; Takashi Mikawa; Terry P Yamaguchi
Journal:  Methods       Date:  2013-06-29       Impact factor: 3.608

6.  Thymosin β4 released from functionalized self-assembling peptide activates epicardium and enhances repair of infarcted myocardium.

Authors:  Yong-Li Wang; Shu-Na Yu; Hao-Ran Shen; Hai-Jie Wang; Xue-Ping Wu; Qiang-Li Wang; Bin Zhou; Yu-Zhen Tan
Journal:  Theranostics       Date:  2021-02-20       Impact factor: 11.556

7.  Epicardial regeneration is guided by cardiac outflow tract and Hedgehog signalling.

Authors:  Jinhu Wang; Jingli Cao; Amy L Dickson; Kenneth D Poss
Journal:  Nature       Date:  2015-05-04       Impact factor: 49.962

8.  Prokineticin receptor-1 signaling promotes Epicardial to Mesenchymal Transition during heart development.

Authors:  Himanshu Arora; Mounia Boulberdaa; Rehana Qureshi; Verda Bitirim; Adeline Gasser; Nadia Messaddeq; Pascal Dolle; Canan G Nebigil
Journal:  Sci Rep       Date:  2016-05-06       Impact factor: 4.379

9.  Human fetal and adult epicardial-derived cells: a novel model to study their activation.

Authors:  Asja T Moerkamp; Kirsten Lodder; Tessa van Herwaarden; Esther Dronkers; Calinda K E Dingenouts; Fredrik C Tengström; Thomas J van Brakel; Marie-José Goumans; Anke M Smits
Journal:  Stem Cell Res Ther       Date:  2016-11-29       Impact factor: 6.832

10.  CDC42 is required for epicardial and pro-epicardial development by mediating FGF receptor trafficking to the plasma membrane.

Authors:  Jingjing Li; Lianjie Miao; Chen Zhao; Wasay Mohiuddin Shaikh Qureshi; David Shieh; Hua Guo; Yangyang Lu; Saiyang Hu; Alice Huang; Lu Zhang; Chen-Leng Cai; Leo Q Wan; Hongbo Xin; Peter Vincent; Harold A Singer; Yi Zheng; Ondine Cleaver; Zhen-Chuan Fan; Mingfu Wu
Journal:  Development       Date:  2017-05-01       Impact factor: 6.868
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