| Literature DB >> 22222529 |
Yixin Jin1, Yang Liu, Marc Antonyak, Xu Peng.
Abstract
The formation of blood vessel networks is a fundamental event in vertebrate embryo development. Angiogenesis and vasculogenesis are the essential processes in vascular formation. Endothelial cells play a key role during angiogenesis and vasculogenesis, and cultured vascular endothelial cells provide an indispensable model for exploring the molecular mechanisms of angiogenesis and vasculogenesis. In this chapter, we described a protocol using PECAM-1-coated Dynabeads for the isolation of vascular endothelial cells from mouse heart and lung. This method will provide up to 10(7) endothelial cells with high purity (>85%). The isolated endothelial cells retain their in vivo characteristics, such as the expression of the cell surface markers PECAM-1 and ICAM-2.Entities:
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Year: 2012 PMID： 22222529 DOI： 10.1007/978-1-61779-523-7_14
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745