| Literature DB >> 22222524 |
Abstract
Assays of angiogenesis in vitro provide insights into vascular development and are useful in studies of agents that modulate blood vessel formation. This chapter describes techniques to induce angiogenesis-like sprouting from aortic and microvascular explants cultured in 3-dimensional native collagen gels. The chapter focuses on explants derived from mice and use of a miniaturized format that permits efficient utilization of reagents, ease of processing, rapid analysis, and conventional imaging.Entities:
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Year: 2012 PMID： 22222524 PMCID： PMC4136681 DOI： 10.1007/978-1-61779-523-7_9
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745