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Literature DB >> 22221796

Aptamer-DNAzyme hairpins for biosensing of Ochratoxin A.

Cheng Yang¹, Vasilica Lates, Beatriz Prieto-Simón, Jean-Louis Marty, Xiurong Yang.

Abstract

We report an aptasensor for biosensing of Ochratoxin A (OTA) using aptamer-DNAzyme hairpin as biorecognition element. The structure of this engineered nucleic acid includes the horseradish peroxidase (HRP)-mimicking DNAzyme and the OTA specific aptamer sequences. A blocking tail captures a part of these sequences in the stem region of the hairpin. In the presence of OTA, the hairpin is opened due to the formation of the aptamer-analyte complex. As a result, self-assembly of the active HRP-mimicking DNAzyme occurs. The activity of this DNAzyme is linearly correlated with OTA concentration up to 10 nM, showing a limit of detection of 2.5 nM.

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Year: 2011 PMID： 22221796 DOI： 10.1016/j.bios.2011.12.011

Source DB: PubMed Journal: Biosens Bioelectron ISSN： 0956-5663 Impact factor: 10.618

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Cited

23 in total

1. Materiomics for Oral Disease Diagnostics and Personal Health Monitoring: Designer Biomaterials for the Next Generation Biomarkers.

Authors: Wenjun Zhang; Ming L Wang; Sammy Khalili; Steven W Cranford
Journal: OMICS Date: 2016-01

2. Dual flow immunochromatographic assay for rapid and simultaneous quantitative detection of ochratoxin A and zearalenone in corn, wheat, and feed samples.

Authors: Xian Zhang; Ke He; Yun Fang; Tong Cao; Narayan Paudyal; Xiao-Feng Zhang; Hou-Hui Song; Xiao-Liang Li; Wei-Huan Fang
Journal: J Zhejiang Univ Sci B Date: 2018 Nov. Impact factor: 3.066

3. Fluorometric aptamer-based determination of ochratoxin A based on the use of graphene oxide and RNase H-aided amplification.

Authors: Changbei Ma; Kefeng Wu; Han Zhao; Haisheng Liu; Kemin Wang; Kun Xia
Journal: Mikrochim Acta Date: 2018-06-30 Impact factor: 5.833

4. Exonuclease-assisted multicolor aptasensor for visual detection of ochratoxin A based on G-quadruplex-hemin DNAzyme-mediated etching of gold nanorod.

Authors: Xinhui Yu; Yaohui Lin; Xusheng Wang; Liangjun Xu; Zongwen Wang; FengFu Fu
Journal: Mikrochim Acta Date: 2018-04-21 Impact factor: 5.833

5. Recent trends in SELEX technique and its application to food safety monitoring.

Authors: Jingjing Wu; Yingyue Zhu; Feng Xue; Zhanlong Mei; Li Yao; Xin Wang; Lei Zheng; Jian Liu; Guodong Liu; Chifang Peng; Wei Chen
Journal: Mikrochim Acta Date: 2014-04 Impact factor: 5.833

6. Design of a Quencher-Free Fluorescent Aptasensor for Ochratoxin A Detection in Red Wine Based on the Guanine-Quenching Ability.

Authors: Cheng Yang; Fathimath Abbas; Amina Rhouati; Yingying Sun; Xiaolin Chu; Shengnan Cui; Bingbing Sun; Changying Xue
Journal: Biosensors (Basel) Date: 2022-05-05

Aptamer-DNAzyme hairpins for biosensing of Ochratoxin A.

1. Materiomics for Oral Disease Diagnostics and Personal Health Monitoring: Designer Biomaterials for the Next Generation Biomarkers.

2. Dual flow immunochromatographic assay for rapid and simultaneous quantitative detection of ochratoxin A and zearalenone in corn, wheat, and feed samples.

3. Fluorometric aptamer-based determination of ochratoxin A based on the use of graphene oxide and RNase H-aided amplification.

4. Exonuclease-assisted multicolor aptasensor for visual detection of ochratoxin A based on G-quadruplex-hemin DNAzyme-mediated etching of gold nanorod.

5. Recent trends in SELEX technique and its application to food safety monitoring.

6. Design of a Quencher-Free Fluorescent Aptasensor for Ochratoxin A Detection in Red Wine Based on the Guanine-Quenching Ability.

Review 7. Immunochemical methods for ochratoxin A detection: a review.

8. Sensitive colorimetric detection of ochratoxin A by a dual-functional Au/Fe₃O₄ nanohybrid-based aptasensor.

Review 9. Single-Stranded DNA Aptamers against Pathogens and Toxins: Identification and Biosensing Applications.

10. Challenges and opportunities for small molecule aptamer development.