OBJECTIVES: The aim of this work was to investigate the antinociceptive property of α-phellandrene (α-PHE) in experimental nociception models and possible mechanisms involved. METHODS: Mass spectrometry was used to evaluate the purity and molecular mass of α-PHE. Macrophages from mice peritoneal cavity were used in an MTT test. Rodents were used in tests of chemical and mechanical nociception. In the study of the mechanisms, the animals were treated with pharmacological tools and then submitted to the glutamate test. KEY FINDINGS: α-PHE purity was 98.2% and molecular mass 136.1 Da. α-PHE did not show cytotoxicity. In the writhing and capsaicin tests, α-PHE promoted the antinociceptive effect in all evaluated doses (minimum dose 3.125 mg/kg). In the formalin test, α-PHE (50 mg/kg) was effective in inhibiting both phases. In the glutamate test, the monoterpene (12.5 mg/kg) decreased the nociceptive response. In carrageenan-induced hyperalgesia, α-PHE (50 mg/kg) decreased the hypernociception index. In the study of the mechanisms involved, pretreatment with naloxone reversed the α-PHE antinociceptive effect, the same occurred with glibenclamide, l-arginine, atropine and yohimbine. α-PHE did not show muscle relaxant activity or central depressant effects in open field and rota rod tests. CONCLUSIONS: α-PHE has an antinociceptive effect and it possibly involves the glutamatergic, opioid, nitrergic, cholinergic and adrenergic systems.
OBJECTIVES: The aim of this work was to investigate the antinociceptive property of α-phellandrene (α-PHE) in experimental nociception models and possible mechanisms involved. METHODS: Mass spectrometry was used to evaluate the purity and molecular mass of α-PHE. Macrophages from mice peritoneal cavity were used in an MTT test. Rodents were used in tests of chemical and mechanical nociception. In the study of the mechanisms, the animals were treated with pharmacological tools and then submitted to the glutamate test. KEY FINDINGS: α-PHE purity was 98.2% and molecular mass 136.1 Da. α-PHE did not show cytotoxicity. In the writhing and capsaicin tests, α-PHE promoted the antinociceptive effect in all evaluated doses (minimum dose 3.125 mg/kg). In the formalin test, α-PHE (50 mg/kg) was effective in inhibiting both phases. In the glutamate test, the monoterpene (12.5 mg/kg) decreased the nociceptive response. In carrageenan-induced hyperalgesia, α-PHE (50 mg/kg) decreased the hypernociception index. In the study of the mechanisms involved, pretreatment with naloxone reversed the α-PHE antinociceptive effect, the same occurred with glibenclamide, l-arginine, atropine and yohimbine. α-PHE did not show muscle relaxant activity or central depressant effects in open field and rota rod tests. CONCLUSIONS: α-PHE has an antinociceptive effect and it possibly involves the glutamatergic, opioid, nitrergic, cholinergic and adrenergic systems.
Authors: Mariana Martins Gomes Pinheiro; Ana B Miltojević; Niko S Radulović; Ikarastika Rahayu Abdul-Wahab; Fabio Boylan; Patrícia Dias Fernandes Journal: PLoS One Date: 2015-03-25 Impact factor: 3.240
Authors: Flávia Franceli de Brito Passos; Everton Moraes Lopes; Jonas Moura de Araújo; Damião Pergentino de Sousa; Leiz Maria C Veras; José Roberto S A Leite; Fernanda Regina de Castro Almeida Journal: Evid Based Complement Alternat Med Date: 2015-06-11 Impact factor: 2.629
Authors: Lenise de Morais Nogueira; Monalisa Ribeiro da Silva; Simone Maria Dos Santos; Julianna Ferreira Cavalcanti de Albuquerque; Igor Cavalcanti Ferraz; Thaíse Torres de Albuquerque; Carlos Renato França de Carvalho Mota; Renata Mendonça Araújo; Glauce Socorro de Barros Viana; René Duarte Martins; Alexandre Havt; Rafael Matos Ximenes Journal: Evid Based Complement Alternat Med Date: 2015-03-02 Impact factor: 2.629
Authors: Rita de Cássia da Silveira E Sá; Tamires Cardoso Lima; Flávio Rogério da Nóbrega; Anna Emmanuela Medeiros de Brito; Damião Pergentino de Sousa Journal: Int J Mol Sci Date: 2017-12-09 Impact factor: 5.923
Authors: Idris A Oyemitan; Christianah A Elusiyan; Ayoola O Onifade; Moses A Akanmu; Adebola O Oyedeji; Armando G McDonald Journal: Toxicol Rep Date: 2017-07-17