Literature DB >> 2221985

Growth advantage by overexpression of normal Harvey ras proto-oncogene in cultured rat epidermal keratinocytes.

K Yamanishi1, F M Liew, Y Hosokawa, S Kishimoto, H Yasuno.   

Abstract

The ras proto-oncogene is frequently amplified and overexpressed in the hyperproliferative conditions of epidermal keratinocytes. To investigate the effects of its overexpression on the growth of keratinocytes in a model system, we constructed expression vectors for normal human Ha-ras and introduced them into FRSK cells, a fetal rat epidermal keratinocyte cell line. Several clones containing the transfected Ha-ras were isolated, and two of them overexpressed this gene. In these clones DNA synthesis and cell growth were greater than in other clones expressing this gene at low levels. Thus we suggest that overexpression of normal ras gene may provide growth advantage to epidermal keratinocytes.

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Year:  1990        PMID： 2221985     DOI： 10.1007/bf00375728

Source DB:  PubMed          Journal:  Arch Dermatol Res        ISSN： 0340-3696            Impact factor:   3.017


  16 in total

1.  A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples.

Authors:  M A Markwell; S M Haas; L L Bieber; N E Tolbert
Journal:  Anal Biochem       Date:  1978-06-15       Impact factor: 3.365

2.  The ras gene. Transformer and transducer.

Authors:  M R Hanley; T Jackson
Journal:  Nature       Date:  1987 Aug 20-26       Impact factor: 49.962

3.  Normal p21N-ras couples bombesin and other growth factor receptors to inositol phosphate production.

Authors:  M J Wakelam; S A Davies; M D Houslay; I McKay; C J Marshall; A Hall
Journal:  Nature       Date:  1986 Sep 11-17       Impact factor: 49.962

4.  The ras oncogene. A structure and some function.

Authors:  I S Sigal
Journal:  Nature       Date:  1988-04-07       Impact factor: 49.962

5.  Complete nucleotide sequences of the T24 human bladder carcinoma oncogene and its normal homologue.

Authors:  D J Capon; E Y Chen; A D Levinson; P H Seeburg; D V Goeddel
Journal:  Nature       Date:  1983-03-03       Impact factor: 49.962

6.  The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection.

Authors:  C M Gorman; G T Merlino; M C Willingham; I Pastan; B H Howard
Journal:  Proc Natl Acad Sci U S A       Date:  1982-11       Impact factor: 11.205

7.  Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.

Authors:  P J Southern; P Berg
Journal:  J Mol Appl Genet       Date:  1982

8.  Qualitative changes in the biologic characteristics of cultured fetal rat keratinizing epidermal cells during the process of malignant transformation after benzo[a]pyrene treatment.

Authors:  K Indo; H Miyaji
Journal:  J Natl Cancer Inst       Date:  1979-10       Impact factor: 13.506

9.  The cytoplasmic protein GAP is implicated as the target for regulation by the ras gene product.

Authors:  C Calés; J F Hancock; C J Marshall; A Hall
Journal:  Nature       Date:  1988-04-07       Impact factor: 49.962

10.  ras gene Amplification and malignant transformation.

Authors:  S Pulciani; E Santos; L K Long; V Sorrentino; M Barbacid
Journal:  Mol Cell Biol       Date:  1985-10       Impact factor: 4.272
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