Literature DB >> 22219308

An alternative method for cultivation of Lawsonia intracellularis.

Fabio A Vannucci1, Suphot Wattanaphansak, Connie J Gebhart.   

Abstract

An alternative method for the cultivation of Lawsonia intracellularis, an obligate intracellular bacterium and the causative agent of proliferative enteropathy, was developed using an Original Space Bag inflated with a mixture of gas containing 10% hydrogen, 10% carbon dioxide, and 80% nitrogen. The flexibility of this protocol allows the testing of various environmental conditions for static cultivation of this bacterium and the development of diagnostic techniques.

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Year:  2012        PMID： 22219308      PMCID： PMC3295169          DOI： 10.1128/JCM.05976-11

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  10 in total

1.  Sensitive determination of cell number using the CyQUANT cell proliferation assay.

Authors:  L J Jones; M Gray; S T Yue; R P Haugland; V L Singer
Journal:  J Immunol Methods       Date:  2001-08-01       Impact factor: 2.303

2.  Validation of an immunoperoxidase monolayer assay as a serologic test for porcine proliferative enteropathy.

Authors:  Roberto M C Guedes; Connie J Gebhart; John Deen; Nathan L Winkelman
Journal:  J Vet Diagn Invest       Date:  2002-11       Impact factor: 1.279

3.  Evaluation of porcine ileum models of enterocyte infection by Lawsonia intracellularis.

Authors:  Steven McOrist; Connie J Gebhart; Brad T Bosworth
Journal:  Can J Vet Res       Date:  2006-04       Impact factor: 1.310

4.  Intestinal Adenoma in Swine.

Authors:  H E Biester; L H Schwarte
Journal:  Am J Pathol       Date:  1931-03       Impact factor: 4.307

5.  Development of a polymerase chain reaction assay for quantification of Lawsonia intracellularis.

Authors:  Suphot Wattanaphansak; Connie J Gebhart; Janet M Anderson; Randall S Singer
Journal:  J Vet Diagn Invest       Date:  2010-07       Impact factor: 1.279

6.  Intracellular bacteria of porcine proliferative enteropathy: cultivation and maintenance in vitro.

Authors:  G H Lawson; S McOrist; S Jasni; R A Mackie
Journal:  J Clin Microbiol       Date:  1993-05       Impact factor: 5.948

7.  Characterization of Lawsonia intracellularis gen. nov., sp. nov., the obligately intracellular bacterium of porcine proliferative enteropathy.

Authors:  S McOrist; C J Gebhart; R Boid; S M Barns
Journal:  Int J Syst Bacteriol       Date:  1995-10

8.  Microarray analysis of differential expression of cell cycle and cell differentiation genes in cells infected with Lawsonia intracellularis.

Authors:  Yu-Sik Oh; Joong-Bok Lee; Steven McOrist
Journal:  Vet J       Date:  2009-04-10       Impact factor: 2.688

9.  Preparation and characterization of polyclonal and monoclonal antibodies against Lawsonia intracellularis.

Authors:  Roberto M C Guedes; Connie J Gebhart
Journal:  J Vet Diagn Invest       Date:  2003-09       Impact factor: 1.279

10.  Adult palatum as a novel source of neural crest-related stem cells.

Authors:  Darius Widera; Christin Zander; Meike Heidbreder; Yvonne Kasperek; Thomas Noll; Oliver Seitz; Belma Saldamli; Holger Sudhoff; Robert Sader; Christian Kaltschmidt; Barbara Kaltschmidt
Journal:  Stem Cells       Date:  2009-08       Impact factor: 6.277
  10 in total
  8 in total

Review 1.  Molecular Hydrogen Metabolism: a Widespread Trait of Pathogenic Bacteria and Protists.

Authors:  Stéphane L Benoit; Chris Greening; Robert J Maier; R Gary Sawers
Journal:  Microbiol Mol Biol Rev       Date:  2020-01-29       Impact factor: 11.056

2.  Comparative transcriptional analysis of homologous pathogenic and non-pathogenic Lawsonia intracellularis isolates in infected porcine cells.

Authors:  Fabio A Vannucci; Douglas N Foster; Connie J Gebhart
Journal:  PLoS One       Date:  2012-10-03       Impact factor: 3.240

3.  Laser microdissection coupled with RNA-seq analysis of porcine enterocytes infected with an obligate intracellular pathogen (Lawsonia intracellularis).

Authors:  Fabio A Vannucci; Douglas N Foster; Connie J Gebhart
Journal:  BMC Genomics       Date:  2013-06-24       Impact factor: 3.969

4.  Probiotics mediated gut microbiota diversity shifts are associated with reduction in histopathology and shedding of Lawsonia intracellularis.

Authors:  Adrian Muwonge; Anbu K Karuppannan; Tanja Opriessnig
Journal:  Anim Microbiome       Date:  2021-03-04

5.  Survival of Lawsonia intracellularis in porcine peripheral blood monocyte-derived macrophages.

Authors:  Carlos Eduardo Real Pereira; Talita Pilar Resende; Aníbal G Armién; Ricardo Pereira Laub; Fabio Augusto Vannucci; Renato Lima Santos; Connie Jane Gebhart; Roberto Mauricio Carvalho Guedes
Journal:  PLoS One       Date:  2020-07-31       Impact factor: 3.240

6.  Metagenomic sequencing of clinical samples reveals a single widespread clone of Lawsonia intracellularis responsible for porcine proliferative enteropathy.

Authors:  Rebecca J Bengtsson; Bryan A Wee; Gonzalo Yebra; Rodrigo Bacigalupe; Eleanor Watson; Roberto M C Guedes; Magdalena Jacobson; Tomasz Stadejek; Alan L Archibald; J Ross Fitzgerald; Tahar Ait-Ali
Journal:  Microb Genom       Date:  2020-04-02

7.  Isolation and in vitro antimicrobial susceptibility of porcine Lawsonia intracellularis from Brazil and Thailand.

Authors:  Suphot Wattanaphansak; Carlos Eduardo Real Pereira; Wenika Kaenson; Pornchalit Assavacheep; Rachod Tantilertcharoen; Talita Pilar Resende; Javier Alberto Barrera-Zarate; Juliana Saes Vilaça de Oliveira-Lee; Ulrich Klein; Connie Jane Gebhart; Roberto Maurício Carvalho Guedes
Journal:  BMC Microbiol       Date:  2019-01-31       Impact factor: 3.605

8.  Evaluation of swine enteroids as in vitro models for Lawsonia intracellularis infection1,2.

Authors:  Talita Pilar Resende; Ramya Lekha Medida; Fabio A Vannucci; Milena Saqui-Salces; Connie Gebhart
Journal:  J Anim Sci       Date:  2020-02-01       Impact factor: 3.159
  8 in total

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