Literature DB >> 22218929

Migration of intervertebral disc cells into dense collagen scaffolds intended for functional replacement.

Johannes L Bron1, Harriet W Mulder, Lucienne A Vonk, Bherouz Zandieh Doulabi, M J Oudhoff, Theodoor H Smit.   

Abstract

Invasion of cells from surrounding tissues is a crucial step for regeneration when using a-cellular scaffolds as a replacement of the nucleus pulposus (NP). The aim of current study was to assess whether NP and surrounding annulus fibrosus (AF) cells are capable of migrating into dense collagen scaffolds. We seeded freshly harvested caprine NP and AF cells onto scaffolds consisting of 1.5 and 3.0% type I collagen matrices, prepared by plastic compression, to assess cell invasion. The migration distance appeared both time and density dependent and was higher for NP (25%) compared to AF (10%) cells after 4 weeks. Migration distance was not enhanced by Hst-2, a peptide derived from saliva known to enhance fibroblast migration, and this was confirmed in a scratch assay. In conclusion, we revealed invasion of cells into dense collagen scaffolds and therewith encouraging first steps towards the use of a-cellular scaffolds for NP replacement.

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Year:  2012        PMID： 22218929      PMCID： PMC3299969          DOI： 10.1007/s10856-011-4545-7

Source DB:  PubMed          Journal:  J Mater Sci Mater Med        ISSN： 0957-4530            Impact factor:   3.896


  30 in total

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Journal:  Cell Biol Int       Date:  2007-09-07       Impact factor: 3.612

2.  Defining the role of matrix compliance and proteolysis in three-dimensional cell spreading and remodeling.

Authors:  Daniel Dikovsky; Havazelet Bianco-Peled; Dror Seliktar
Journal:  Biophys J       Date:  2008-01-04       Impact factor: 4.033

3.  Rheological characterization of the nucleus pulposus and dense collagen scaffolds intended for functional replacement.

Authors:  J L Bron; G H Koenderink; V Everts; T H Smit
Journal:  J Orthop Res       Date:  2009-05       Impact factor: 3.494

4.  Identification and characterization of the endocytic transmembrane glycoprotein Endo180 as a novel collagen receptor.

Authors:  Dirk Wienke; John R MacFadyen; Clare M Isacke
Journal:  Mol Biol Cell       Date:  2003-07-25       Impact factor: 4.138

Review 5.  Fibroblasts in three dimensional matrices: cell migration and matrix remodeling.

Authors:  Sangmyung Rhee
Journal:  Exp Mol Med       Date:  2009-12-31       Impact factor: 8.718

6.  Histatins enhance wound closure with oral and non-oral cells.

Authors:  M J Oudhoff; P A M van den Keijbus; K L Kroeze; K Nazmi; S Gibbs; J G M Bolscher; E C I Veerman
Journal:  J Dent Res       Date:  2009-09       Impact factor: 6.116

Review 7.  Injectable biomaterials and vertebral endplate treatment for repair and regeneration of the intervertebral disc.

Authors:  Lawrence M Boyd; Andrew J Carter
Journal:  Eur Spine J       Date:  2006-07-26       Impact factor: 3.134

8.  Caprine articular, meniscus and intervertebral disc cartilage: an integral analysis of collagen network and chondrocytes.

Authors:  Lucienne A Vonk; Robert Jan Kroeze; Behrouz Zandieh Doulabi; Roel J Hoogendoorn; Chunling Huang; Marco N Helder; Vincent Everts; Ruud A Bank
Journal:  Matrix Biol       Date:  2010-01-03       Impact factor: 11.583

9.  Differential phenotype of intervertebral disc cells: microarray and immunohistochemical analysis of canine nucleus pulposus and anulus fibrosus.

Authors:  Daisuke Sakai; Tomoko Nakai; Joji Mochida; Mauro Alini; Sibylle Grad
Journal:  Spine (Phila Pa 1976)       Date:  2009-06-15       Impact factor: 3.468

10.  Reoperations after first lumbar disc herniation surgery; a special interest on residives during a 5-year follow-up.

Authors:  Arja Häkkinen; Ilkka Kiviranta; Marko H Neva; Hannu Kautiainen; Jari Ylinen
Journal:  BMC Musculoskelet Disord       Date:  2007-01-09       Impact factor: 2.362
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  6 in total

1.  Injectable, high-density collagen gels for annulus fibrosus repair: An in vitro rat tail model.

Authors:  Brandon Borde; Peter Grunert; Roger Härtl; Lawrence J Bonassar
Journal:  J Biomed Mater Res A       Date:  2014-12-29       Impact factor: 4.396

2.  Dental pulp stem cell-derived chondrogenic cells demonstrate differential cell motility in type I and type II collagen hydrogels.

Authors:  Li Yao; Nikol Flynn
Journal:  Spine J       Date:  2018-02-13       Impact factor: 4.166

3.  Fabrication of dense anisotropic collagen scaffolds using biaxial compression.

Authors:  Jared L Zitnay; Shawn P Reese; Garvin Tran; Niloofar Farhang; Robert D Bowles; Jeffrey A Weiss
Journal:  Acta Biomater       Date:  2017-11-08       Impact factor: 8.947

4.  Periodic mechanical stress induces the extracellular matrix expression and migration of rat nucleus pulposus cells by upregulating the expression of intergrin α1 and phosphorylation of downstream phospholipase Cγ1.

Authors:  Gongming Gao; Jin He; Luming Nong; Hua Xie; Yongjing Huang; Nanwei Xu; Dong Zhou
Journal:  Mol Med Rep       Date:  2016-07-27       Impact factor: 2.952

Review 5.  Advances of Naturally Derived and Synthetic Hydrogels for Intervertebral Disk Regeneration.

Authors:  Guoke Tang; Bingyan Zhou; Feng Li; Weiheng Wang; Yi Liu; Xing Wang; Chao Liu; Xiaojian Ye
Journal:  Front Bioeng Biotechnol       Date:  2020-06-30

Review 6.  Biomaterials and Cell-Based Regenerative Therapies for Intervertebral Disc Degeneration with a Focus on Biological and Biomechanical Functional Repair: Targeting Treatments for Disc Herniation.

Authors:  Katsuhisa Yamada; Norimasa Iwasaki; Hideki Sudo
Journal:  Cells       Date:  2022-02-09       Impact factor: 6.600
  6 in total

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