Literature DB >> 2221377

Detection of lipopolysaccharides blotted to polyvinylidene difluoride membranes.

M A Stein1, S A McAllister, K H Johnston, D L Diedrich.   

Abstract

Bacterial lipopolysaccharide (LPS) blotted to polyvinylidene difluoride (PVDF) membranes was detected by a technique adapted from current methodologies used to detect glycoproteins. PVDF-bound LPS was coupled to a hapten and localized on the membrane by Western blotting with an antibody-alkaline phosphatase conjugate specific for the hapten. Immobilon blots could be made reversibly transparent for photography and densitometry.

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Year:  1990        PMID： 2221377     DOI： 10.1016/0003-2697(90)90607-b

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  3 in total

1.  Acquisition of apparently intact and unmodified lipopolysaccharides from Escherichia coli by Bdellovibrio bacteriovorus.

Authors:  M A Stein; S A McAllister; B E Torian; D L Diedrich
Journal:  J Bacteriol       Date:  1992-05       Impact factor: 3.490

2.  Salmonella serogroups C2 and C3 identified by agglutination using an immunoglobulin G3(kappa) monoclonal antibody (32-1-E3) reactive with a somatic factor 8-like polysaccharide antigen.

Authors:  P S Duffey; J C Kani; J O Lee; W J Hill; R Kokka
Journal:  J Clin Microbiol       Date:  1992-12       Impact factor: 5.948

3.  Optimization of alginate purification using polyvinylidene difluoride membrane filtration: Effects on immunogenicity and biocompatibility of three-dimensional alginate scaffolds.

Authors:  Hugo P Sondermeijer; Piotr Witkowski; David Woodland; Tetsunori Seki; Frank J Aangenendt; Arnoud van der Laarse; Silviu Itescu; Mark A Hardy
Journal:  J Biomater Appl       Date:  2016-04-25       Impact factor: 2.646
  3 in total

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