Literature DB >> 22212760

Evaluation of propidium monoazide (PMA) treatment directly on membrane filter for the enumeration of viable but non cultivable Legionella by qPCR.

Sami Slimani1, Audrey Robyns, Sophie Jarraud, Maëlle Molmeret, Eric Dusserre, Céline Mazure, Jean Pierre Facon, Gérard Lina, Jerome Etienne, Christophe Ginevra.   

Abstract

A PMA (propidium monoazide) pretreatment protocol, in which PMA is applied directly to membrane filters, was developed for the PCR-based quantification (PMA-qPCR) of viable Legionella pneumophila. Using this method, the amplification of DNA from membrane-damaged L. pneumophila was strongly inhibited for samples containing a small number of dead bacteria.
Copyright © 2011 Elsevier B.V. All rights reserved.

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Year:  2011        PMID： 22212760     DOI： 10.1016/j.mimet.2011.12.010

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  13 in total

1.  On-filter direct amplification of Legionella pneumophila for rapid assessment of its abundance and viability.

Authors:  Farag A Samhan; Tiffany M Stedtfeld; Hassan Waseem; Maggie R Williams; Robert D Stedtfeld; Syed A Hashsham
Journal:  Water Res       Date:  2017-05-13       Impact factor: 11.236

2.  Molecular survey of the occurrence of Legionella spp., Mycobacterium spp., Pseudomonas aeruginosa, and amoeba hosts in two chloraminated drinking water distribution systems.

Authors:  Hong Wang; Marc Edwards; Joseph O Falkinham; Amy Pruden
Journal:  Appl Environ Microbiol       Date:  2012-06-29       Impact factor: 4.792

Review 3.  Methodological approaches for monitoring opportunistic pathogens in premise plumbing: A review.

Authors:  Hong Wang; Emilie Bédard; Michèle Prévost; Anne K Camper; Vincent R Hill; Amy Pruden
Journal:  Water Res       Date:  2017-03-25       Impact factor: 11.236

4.  Legionella: A Promising Supplementary Indicator of Microbial Drinking Water Quality in Municipal Engineered Water Systems.

Authors:  Chiqian Zhang; Jingrang Lu
Journal:  Front Environ Sci       Date:  2021-11-10

5.  A novel method for rapid and sensitive detection of viable Escherichia coli cells using UV-induced PMA-coupled quantitative PCR.

Authors:  Rehan Deshmukh; Sunil Bhand; Utpal Roy
Journal:  Braz J Microbiol       Date:  2019-10-26       Impact factor: 2.476

Review 6.  The many forms of a pleomorphic bacterial pathogen-the developmental network of Legionella pneumophila.

Authors:  Peter Robertson; Hany Abdelhady; Rafael A Garduño
Journal:  Front Microbiol       Date:  2014-12-22       Impact factor: 5.640

7.  Molecular characterization of viable Legionella spp. in cooling tower water samples by combined use of ethidium monoazide and PCR.

Authors:  Hiroaki Inoue; Reiko Fujimura; Kunio Agata; Hiroyuki Ohta
Journal:  Microbes Environ       Date:  2015-01-16       Impact factor: 2.912

Review 8.  Determination of viable legionellae in engineered water systems: Do we find what we are looking for?

Authors:  Alexander K T Kirschner
Journal:  Water Res       Date:  2016-02-12       Impact factor: 11.236

Review 9.  Pathogens protection against the action of disinfectants in multispecies biofilms.

Authors:  Pilar Sanchez-Vizuete; Belen Orgaz; Stéphane Aymerich; Dominique Le Coq; Romain Briandet
Journal:  Front Microbiol       Date:  2015-07-14       Impact factor: 5.640

10.  Management of the 2012 Legionella crisis in Quebec City: need for a better communication between resources and knowledge transfer.

Authors:  Luc Trudel; Marc Veillette; Laetitia Bonifait; Caroline Duchaine
Journal:  Front Microbiol       Date:  2014-05-05       Impact factor: 5.640
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