Literature DB >> 22211021

Electrical conductivity as a tool to detect salt in clinical proteomics samples.

Gururao Hariprasad1, Alagiri Srinivasan, Yadagiri Vedakumari.   

Abstract

Clinical proteomics encompasses the study of the proteins in the human body at different settings to understand the various physiological and pathological pathways. The processing of the samples for electrophoresis based proteomics is a challenge to any researcher. Salt in particular can have an array of effects during the electrophoretic separation of proteins. There is a definite need to determine the concentration of salts in the samples and the effectiveness of salt removing protocols on small volume samples. A simple-cost effective technique to know the salt concentration in the clinical proteomics samples has been highlighted in the report. The application will be of value in a developing country such as India.

Entities:  

Keywords:  2D electrophoresis; Clinical proteomics; Salt

Year:  2010        PMID: 22211021      PMCID: PMC3068766          DOI: 10.1007/s12291-010-0086-y

Source DB:  PubMed          Journal:  Indian J Clin Biochem        ISSN: 0970-1915


  2 in total

1.  A simple, inexpensive method for the salt-free concentration of proteins for analysis by two-dimensional gel electrophoresis.

Authors:  G A Maresh; B S Dunbar
Journal:  Electrophoresis       Date:  1988-01       Impact factor: 3.535

2.  Effect of salt concentration on separation patterns in static capillary isoelectric focusing with imaging detection.

Authors:  Q Mao; J Pawliszyn
Journal:  J Chromatogr B Biomed Sci Appl       Date:  1999-06-11
  2 in total
  2 in total

1.  Proteomics of Sentinel Lymph Nodes in Early Breast Cancer for Identification of Thymidylate Synthase as a Potential Biomarker to Flag Metastasis: A Preliminary Study.

Authors:  Sheetal Pathania; Mohd Imran Khan; Abhishek Kumar; Ashish Kumar Gupta; Komal Rani; Tanvi Ramesh Parashar; Jnaneshwari Jayaram; Piyush Ranjan Mishra; Anurag Srivastava; Sandeep Mathur; Smriti Hari; Gururao Hariprasad
Journal:  Cancer Manag Res       Date:  2020-06-23       Impact factor: 3.989

2.  Comparative Proteomic Analysis of Advanced Ovarian Cancer Tissue to Identify Potential Biomarkers of Responders and Nonresponders to First-Line Chemotherapy of Carboplatin and Paclitaxel.

Authors:  Urmila Sehrawat; Ruchika Pokhriyal; Ashish Kumar Gupta; Roopa Hariprasad; Mohd Imran Khan; Divya Gupta; Jasmine Naru; Sundararajan Baskar Singh; Ashok Kumar Mohanty; Perumal Vanamail; Lalit Kumar; Sunesh Kumar; Gururao Hariprasad
Journal:  Biomark Cancer       Date:  2016-03-16
  2 in total

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