OBJECTIVE: The aim of our study was to investigate the frequency and compare the prevalence of IRS-1Gly972Arg and IRS-2 Gly1057Asp polymorphisms in PCOS patients and non-diabetic healthy women. MATERIAL(S) AND METHOD(S): Forty eight Iranian women diagnosed with PCOS were enrolled in this study. Fifty two non-diabetics, non-PCOS women were enrolled as the control group. HemoglobinA1c (HbA1c), fasting blood glucose (FBS), fasting insulin levels (FIL) and 2 h post-prandial blood glucose(2hpp BS) were evaluated from blood samples. Insulin resistance sample was estimated with Homeostasis Model Assessment index for insulin resistance (HOMA-IR). Genotyping of allelic variants Gly972Arg of IRS-1 and Gly1057Asp of IRS-2 was conducted using PCR. RESULTS: No statistically significant differences in the prevalence of IRS-1 Gly972Arg or IRS-2 Gly1057Asp polymorphisms or any combination of both were observed between controls and PCOS patients (P > 0.02). Control subjects with the IRS-1 polymorphism had higher levels of 2hpp BS compared with those with the Gly/Gly genotype (P = 0.037). CONCLUSIONS: Considering that no association between the IRS-1 Gly972Arg and IRS-2 Gly1057Asp polymorphisms and PCOS were found, the results confirm that these polymorphisms should not be considered as major contributors to the pathogenesis of this disorder.
OBJECTIVE: The aim of our study was to investigate the frequency and compare the prevalence of IRS-1Gly972Arg and IRS-2 Gly1057Asp polymorphisms in PCOSpatients and non-diabetic healthy women. MATERIAL(S) AND METHOD(S): Forty eight Iranian women diagnosed with PCOS were enrolled in this study. Fifty two non-diabetics, non-PCOSwomen were enrolled as the control group. HemoglobinA1c (HbA1c), fasting blood glucose (FBS), fasting insulin levels (FIL) and 2 h post-prandial blood glucose(2hpp BS) were evaluated from blood samples. Insulin resistance sample was estimated with Homeostasis Model Assessment index for insulin resistance (HOMA-IR). Genotyping of allelic variants Gly972Arg of IRS-1 and Gly1057Asp of IRS-2 was conducted using PCR. RESULTS: No statistically significant differences in the prevalence of IRS-1Gly972Arg or IRS-2 Gly1057Asp polymorphisms or any combination of both were observed between controls and PCOSpatients (P > 0.02). Control subjects with the IRS-1 polymorphism had higher levels of 2hpp BS compared with those with the Gly/Gly genotype (P = 0.037). CONCLUSIONS: Considering that no association between the IRS-1Gly972Arg and IRS-2 Gly1057Asp polymorphisms and PCOS were found, the results confirm that these polymorphisms should not be considered as major contributors to the pathogenesis of this disorder.
Authors: S A El Mkadem; C Lautier; F Macari; N Molinari; P Lefèbvre; E Renard; J C Gris; G Cros; J P Daurès; J Bringer; M F White; F Grigorescu Journal: Diabetes Date: 2001-09 Impact factor: 9.461
Authors: M A Pappalardo; G T Russo; A Pedone; A Pizzo; I Borrielli; G Stabile; A C Artenisio; A Amato; M Calvani; D Cucinotta; F Trimarchi; S Benvenga Journal: Horm Metab Res Date: 2010-03-12 Impact factor: 2.936
Authors: M L Hribal; M Federici; O Porzio; D Lauro; P Borboni; D Accili; R Lauro; G Sesti Journal: J Clin Endocrinol Metab Date: 2000-05 Impact factor: 5.958