Characterization of the lung epithelium of wild-type and TLR9(-/-) mice after single and repeated exposures to chicken barn air.

Exposure to chicken barn air causes lung injury resulting in lower and upper respiratory symptoms in the poultry workers, and mechanisms of which are not fully understood. The lung injury can initiate modifications such as proliferation of the airway epithelial cells such as Clara cells, type II alveolar (T2) cells and mucus producing goblet cells as part of the innate immune response. Toll-like receptors (TLR) have been suggested to play a role in cell division and proliferation. To understand the effect of TLR9 on Clara cells, T2 and mucus-producing goblet cells, we quantified the numbers of these cells in the lungs of wild-type (WT) and TLR9(-/-) mice exposed to chicken barn air. The mice were exposed for either one day or five or 20 days for 8 h/day. Clara cells and T2 cells were labelled with antibodies, and the mucus cells were identified with Periodic-acid Schiff stain, and quantified in per unit tissue section area. The data show decrease in the number of Clara cells and increase in mucus-producing goblet cells after exposure to chicken barn air in both WT and TLR9(-/-) mice. Numbers of T2 cells increased and decreased in WT and TLR9(-/-) mice, respectively, after exposure to poultry barn air. These data show that exposure to chicken barn air can affect major lung epithelial cells, and allude to the role of TLR9 in regulation of some of these responses.