PREMISE OF THE STUDY: Date palm (Phoenix dactylifera) is one of the oldest cultivated trees and is critical to the development of arid land. The date palm is a dioecious monocot with separate male and female trees. This presents a challenge in development as it is impossible to distinguish trees until they flower approximately five to eight years after planting. METHODS AND RESULTS: We have developed PCR-based assays capable of sex differentiation in multiple date palm cultivars. The primers are designed across gender-specific polymorphisms and demonstrated greater than 90% accuracy in distinguishing date palm gender across multiple varieties. CONCLUSIONS: These results indicate that the primers should be helpful in rapidly distinguishing date palm gender from the earliest stages that DNA can safely be collected. This is a vast savings in time over present approaches.
PREMISE OF THE STUDY: Date palm (Phoenix dactylifera) is one of the oldest cultivated trees and is critical to the development of arid land. The date palm is a dioecious monocot with separate male and female trees. This presents a challenge in development as it is impossible to distinguish trees until they flower approximately five to eight years after planting. METHODS AND RESULTS: We have developed PCR-based assays capable of sex differentiation in multiple date palm cultivars. The primers are designed across gender-specific polymorphisms and demonstrated greater than 90% accuracy in distinguishing date palm gender across multiple varieties. CONCLUSIONS: These results indicate that the primers should be helpful in rapidly distinguishing date palm gender from the earliest stages that DNA can safely be collected. This is a vast savings in time over present approaches.
Authors: Jamal S M Sabir; Dhivya Arasappan; Ahmed Bahieldin; Salah Abo-Aba; Sameera Bafeel; Talal A Zari; Sherif Edris; Ahmed M Shokry; Nour O Gadalla; Ahmed M Ramadan; Ahmed Atef; Magdy A Al-Kordy; Fotoh M El-Domyati; Robert K Jansen Journal: PLoS One Date: 2014-04-09 Impact factor: 3.240
Authors: Maria F Torres; Lisa S Mathew; Ikhlak Ahmed; Iman K Al-Azwani; Robert Krueger; Diego Rivera-Nuñez; Yasmin A Mohamoud; Andrew G Clark; Karsten Suhre; Joel A Malek Journal: Nat Commun Date: 2018-09-28 Impact factor: 14.919
Authors: Kareem A Mosa; Sanjay Gairola; Rahul Jamdade; Ali El-Keblawy; Khawla Ibrahim Al Shaer; Eman Khalid Al Harthi; Hatem A Shabana; Tamer Mahmoud Journal: Front Plant Sci Date: 2019-01-21 Impact factor: 5.753