Some comments on recent discussion of the Boyle van't Hoff relationship.

The estimation of several cellular biophysical parameters must be made in order to mathematically predict optimal cryopreservation protocols. These parameters include total cell volume, osmotically inactive volume, cell surface area, and relative water and solute permeabilities. Recent attention has been paid to the determination of the osmotically inactive volume and, specifically, an argument was made suggesting that this volume was incorrectly determined in the literature [4]. Here we show that this assertion is false.