Hadi Hassannia1, Saeid Abediankenari, Javad Ghaffari. 1. Department of Immunology and Microbiology, Mazandaran University of Medical Sciences, Sari, Iran, e-mail: abedianks@razi.tums.ac.ir.
Abstract
BACKGROUND: Regulatory CD4+ T (Treg) cells are effective in maintaining immune tolerance. OBJECTIVE: To investigate single nucleotide polymorphisms (SNPs) of Transforming Growth Factor β-1 (TGF-β1) and Forkhead Box Protein 3 (FOXP3) genes in Iranian patients with allergic rhinitis (AR). METHODS: Variations at codons 10 and 25 of TGF-β1 and FOXP3 at positions -3279 A>C and -924 A>G were evaluated in AR patients and compared with controls. In a case-control study, 155 AR patients and 163 allergy-free controls were genotyped using polymerase chain reaction sequence-specific primer (PCR-SSP) technique. RESULTS: The analysis of the frequency of these SNPs showed that the haplotype formed by FOXP3 -3279 A allele occurred significantly more frequently in patients than controls (odds ratio=1.44, 95% CI=1.312-2.66; p=0.001). CONCLUSION: Our results suggest that polymorphism in FOXP3 gene is associated with susceptibility to AR.
BACKGROUND: Regulatory CD4+ T (Treg) cells are effective in maintaining immune tolerance. OBJECTIVE: To investigate single nucleotide polymorphisms (SNPs) of Transforming Growth Factor β-1 (TGF-β1) and Forkhead Box Protein 3 (FOXP3) genes in Iranian patients with allergic rhinitis (AR). METHODS: Variations at codons 10 and 25 of TGF-β1 and FOXP3 at positions -3279 A>C and -924 A>G were evaluated in AR patients and compared with controls. In a case-control study, 155 AR patients and 163 allergy-free controls were genotyped using polymerase chain reaction sequence-specific primer (PCR-SSP) technique. RESULTS: The analysis of the frequency of these SNPs showed that the haplotype formed by FOXP3 -3279 A allele occurred significantly more frequently in patients than controls (odds ratio=1.44, 95% CI=1.312-2.66; p=0.001). CONCLUSION: Our results suggest that polymorphism in FOXP3 gene is associated with susceptibility to AR.
Authors: Cintia Rodrigues Marques; Ryan Santos Costa; Gustavo Nunes de Oliveira Costa; Thiago Magalhães da Silva; Tatiane Oliveira Teixeira; Emília Maria Medeiros de Andrade; Alana A Galvão; Valdirene Leão Carneiro; Camila Alexandrina Figueiredo Journal: Asthma Res Pract Date: 2015-10-20